ArticleBiomedical optics express2021
Real-time pixelwise phasor analysis for video-rate two-photon fluorescence lifetime imaging microscopy.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The trial behind it
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Who cites it
23 citing papers in PubMed, 26 citations in OpenAlex.
- Impact of noise on optical redox ratio estimation.bioRxiv : the preprint server for biology · 2026Article
- Phasor EO-FLIM: Lifetime imaging with picosecond noise and 500 Hz frame rate.bioRxiv : the preprint server for biology · 2026Article
- Label-free pathological subtyping of non-small cell lung cancer using deep classification and virtual immunohistochemical staining.NPJ digital medicine · 2026Article
- Brain-SAM: a general automatic SAM-based segmentation model for brain science images.Biomedical optics express · 2026Article
- Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.Neurophotonics · 2026Article
- Artificial Intelligence in Label-Free Optical Imaging Applications.Methods in molecular biology (Clifton, N.J.) · 2026Article
- On the importance of simultaneous label-free multimodal nonlinear optical imaging for biomedical applications.APL photonics · 2025Article
- Deep Learning for Fluorescence Lifetime Predictions Enables High-Throughput In Vivo Imaging.Journal of the American Chemical Society · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Multiphoton microscopy at a microwatt level via gain-managed nonlinear amplification and pulse-picking.Biomedical optics express · 2025Article
- Label-free metabolic fingerprinting of motile mammalian spermatozoa with subcellular resolution.BMC biology · 2025Article
- Fluorescence Lifetime Multiplexing (FLEX) for simultaneous high dimensional spatial biology in 3D.Communications biology · 2024Article
- Inspiring a convergent engineering approach to measure and model the tissue microenvironment.Heliyon · 2024Review
- Analog multiplexing of a laser clock and computational photon counting for fast fluorescence lifetime imaging microscopy.Biomedical optics express · 2024Article
- Article
- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- FLUTE: A Python GUI for interactive phasor analysis of FLIM data.Biological imaging · 2023Article
- Identifying lipid particle sub-types in liveFrontiers in chemistry · 2023Article
- Real-time open-source FLIM analysis.Frontiers in bioinformatics · 2023Article
- Tracking the binding of multi-functional fluorescent tags for Alzheimer's disease using quantitative multiphoton microscopy.Journal of biophotonics · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Two-photon fluorescence lifetime imaging microscopy (FLIM) is a widely used technique in biomedical optical imaging. Presently, many two-photon time-domain FLIM setups are limited by long acquisition and postprocessing times that decrease data throughput and inhibit the ability to image fast sub-second processes. Here, we present a versatile two-photon FLIM setup capable of video-rate (up to 25 fps) imaging with graphics processing unit (GPU)-accelerated pixelwise phasor analysis displayed and saved simultaneously with acquisition. The system uses an analog output photomultiplier tube in conjunction with 12-bit digitization at 3.2 GHz to overcome the limited maximum acceptable photon rate associated with the photon counting electronics in many FLIM systems. This allows for higher throughput FLIM acquisition and analysis, and additionally enables the user to assess sample fluorescence lifetime in real-time. We further explore the capabilities of the system to examine the kinetics of Rhodamine B uptake by human breast cancer cells and characterize the effect of pixel dwell time on the reduced nicotinamide adenine dinucleotide and reduced nicotinamide adenine dinucleotide phosphate (NAD(P)H) autofluorescence lifetime estimation accuracy.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.