ArticleBiomedical optics express2021
Biochemical resolving power of fluorescence lifetime imaging: untangling the roles of the instrument response function and photon-statistics.
Article in Biomedical optics express, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2026Article
- FLIM quality metric visualization as a means to validate consistency across large-area non-homogeneous FLIM datasets.Methods and applications in fluorescence · 2026Article
- Label-free nonlinear microscopy probes cellular metabolism and myelin dynamics in live tissue.Communications biology · 2025Article
- Segmentation-guided photon pooling enables robust single cell analysis and fast fluorescence lifetime imaging microscopy.bioRxiv : the preprint server for biology · 2025Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.eLife · 2025Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.bioRxiv : the preprint server for biology · 2025Article
- A low-cost FPGA-based approach for pile-up corrected high-speedNeurophotonics · 2025Article
- Calibration approaches for fluorescence lifetime applications using time-domain measurements.Methods in microscopy · 2025Article
- Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling.Journal of biomedical optics · 2025Article
- Low-Temperature Emission Dynamics of Methylammonium Lead Bromide Hybrid Perovskite Thin Films at the Sub-Micrometer Scale.Nanomaterials (Basel, Switzerland) · 2023Article
- Design and characterization of a time-domain optical tomography platform for mesoscopic lifetime imaging.Biomedical optics express · 2022Article
- Controlled Synthesis of Luminescent Xanthene Dyes and Use of Ionic Liquid in Thermochromic Reaction.Molecules (Basel, Switzerland) · 2022Article
- Article
- Advanced Data Analysis for Fluorescence-Lifetime Single-Molecule Localization Microscopy.Frontiers in bioinformatics · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A deeper understanding of spatial resolution has led to innovations in microscopy and the disruption of biomedical research, as with super-resolution microscopy. To foster similar advances in time-resolved and spectral imaging, we have previously introduced the concept of 'biochemical resolving power' in fluorescence microscopy. Here, we apply those concepts to investigate how the instrument response function (IRF), sampling conditions, and photon-statistics limit the biochemical resolution of fluorescence lifetime microscopy. Using Fisher information analysis and Monte Carlo simulations, we reveal the complex dependencies between photon-statistics and the IRF, permitting us to quantify resolution limits that have been poorly understood (
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Registered trials
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