Evidence map›Paper›PMID 34454988›Full record

ArticleJournal of virological methods2021

A rapid and simple protocol for concentration of SARS-CoV-2 from sewage.

Diana P Wehrendt, Mariana G Massó, Adrián Gonzales Machuca, Claudia V Vargas, Melina E Barrios, Josefina Campos, Damián Costamagna, Luis Bruzzone, Daniel M Cisterna, Néstor Gabriel Iglesias and 5 more

Open access · greenAbstract read
In one paragraph

Article in Journal of virological methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Review
  5. Presence of SARS-CoV-2 in urban effluents in south-east Buenos Aires, Argentina, May 2020 to March 2022.Revista panamericana de salud publica = Pan American journal of public health · 2023
    Article
  6. Review
  7. A reduced graphene oxide-FeChemosphere · 2022
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 6 institutions in 1 country.

Diana P WehrendtInstituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr Héctor N. Torres" (INGEBI) Vuelta de Obligado 2490, C1428ADN, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Mariana G MassóLaboratorio de Investigaciones en Mecanismos de Resistencia a Antibióticos. Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires (IMPaM, UBA-CONICET), Argentina.
Adrián Gonzales MachucaLaboratorio de Investigaciones en Mecanismos de Resistencia a Antibióticos. Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires (IMPaM, UBA-CONICET), Argentina.
Claudia V VargasInstituto de Investigaciones en Producción Animal (INPA), Argentina.
Melina E BarriosConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Universidad de Buenos Aires, Facultad de Farmacia y Bioquimica, Argentina.
Josefina CamposInstituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr Carlos G. Malbrán, Argentina.
Damián CostamagnaAutoridad del Agua, Gobierno de la Provincia de Buenos Aires, Argentina.
Luis BruzzoneAguas Bonaerenses SA, Argentina.
Daniel M CisternaInstituto Nacional de Enfermedades Infecciosas (INEI) ANLIS "Dr Carlos G. Malbrán, Argentina.
Néstor Gabriel IglesiasConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Laboratorio de Virus Emergentes, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Argentina.
Viviana A MbayedConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Universidad de Buenos Aires, Facultad de Farmacia y Bioquimica, Argentina; Instituto de Investigaciones en Bacteriología y Virologia Molecular (IBAViM), Argentina.
Elsa BaumeisterServicio Virosis Respiratorias, Laboratorio Nacional de Referencia de Enfermedades Respiratorias Virales, INEI-ANLIS "Dr Carlos G. Malbrán", Centro Nacional de Influenza de OMS, Argentina.
Daniela CentrónConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Laboratorio de Investigaciones en Mecanismos de Resistencia a Antibióticos. Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires (IMPaM, UBA-CONICET), Argentina.
María Paula QuirogaConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Laboratorio de Investigaciones en Mecanismos de Resistencia a Antibióticos. Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires (IMPaM, UBA-CONICET), Argentina.
Leonardo ErijmanInstituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr Héctor N. Torres" (INGEBI) Vuelta de Obligado 2490, C1428ADN, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina. Electronic address: erijman@dna.uba.ar.
Consejo Nacional de Investigaciones Científicas y Técnicas · ARAdministración Nacional de Laboratorios e Institutos de Salud · ARUniversidad de Buenos Aires · ARCentro Científico Tecnológico - San Juan · ARMinisterio de Salud - Provincia de Buenos Aires · ARNational University of Quilmes · AR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of this study was to set up a simple protocol to concentrate SARS-CoV-2 from sewage, which can be implemented in laboratories with minimal equipment resources. The method avoids the need for extensive purification steps and reduces the concentration of potential inhibitors of RT-qPCR contained in sewage. The concentration method consists of a single step, in which a small volume (40 mL) of sewage sample is incubated with polyaluminum chloride (PAC)(0.00045 N Al

Indexed as

COVID-19SewageHumansRNA, ViralSARS-CoV-2WastewaterRNA, ViralSewageWastewaterEnvironmental surveillanceSARS-CoV-2SewageVirus concentrationWastewater based epidemiology

Identifiers

PMID34454988
PMCPMC8388153
OpenAlexW3198613205

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.