Evidence map›Paper›PMID 34453719›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Methods for Assessing the Regulation of a Kinase by the Rab GTPase Ypt1.

Juan Wang, Shensen Wang, Susan Ferro-Novick

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Juan WangCollege of Life Science and Bioengineering, Beijing University of Technology, Beijing, People's Republic of China.
Shensen WangCollege of Life Science and Bioengineering, Beijing University of Technology, Beijing, People's Republic of China.
Susan Ferro-NovickDepartment of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA, USA. sfnovick@ucsd.edu.
Beijing University of Technology · CNUniversity of California, San Diego · US

Funding

The diverse roles of ER-Golgi trafficking machinery in autophagy and ER quality controlR35GM131681 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Susan FERRO-NOVICK · 2019 to 2026
$5.5M
The role of a CK1 kinase in membrane trafficR01GM114111 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FERRO-NOVICK, SUSAN · 2015 to 2018
$1.5M
The contribution of the secretory pathway to macroautophagyR01GM115422 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FERRO-NOVICK, SUSAN · 2016 to 2018
$1.1M
NIGMS NIH HHS R01 GM114111NIGMS NIH HHS R01 GM115422NIGMS NIH HHS R35 GM131681
6 · The paper itself

Abstract

COPII coated vesicles that bud from the endoplasmic reticulum (ER) normally traffic to the Golgi. However, during starvation, COPII vesicles are redirected to the macroautophagy pathway where they become a membrane source for autophagosomes. Phosphorylation of the coat by the casein kinase 1 (CK1), Hrr25, is a prerequisite for vesicle uncoating and membrane fusion. CK1 family members were initially thought to be constitutively active kinases that are regulated through their subcellular localization. Recent studies, however, have shown that the Rab GTPase Ypt1 binds to and activates Hrr25 (CK1δ in mammals) to spatially regulate its kinase activity. Consistent with a direct role for Hrr25 in macroautophagy, hrr25and ypt1mutants are defective in autophagosome biogenesis. These studies have provided insights into how the itinerary of COPII vesicles is coordinated on two different trafficking pathways.

Indexed as

Saccharomyces cerevisiaeAnimalsCasein Kinase ICOP-Coated VesiclesGolgi Apparatusrab GTP-Binding ProteinsSaccharomyces cerevisiae ProteinsVesicular Transport ProteinsCasein Kinase IHRR25 protein, S cerevisiaerab GTP-Binding ProteinsSaccharomyces cerevisiae ProteinsVesicular Transport ProteinsYPT1 protein, S cerevisiaeCasein kinaseHrr25Rab GTPaseVesicle trafficYpt1

Identifiers

PMID34453719
PMCPMC8893604
OpenAlexW3195878420

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.