Evidence map›Paper›PMID 34453584›Full record

ArticleAmino acids2022

Characterization of a novel + 70 Da modification in rhGM-CSF expressed in E. coli using chemical assays in combination with mass spectrometry.

Magdalena Widgren Sandberg, Jakob Bunkenborg, Stine Thyssen, Martin Villadsen, Thomas Kofoed

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Amino acids, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Editorial.Amino acids · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Magdalena Widgren SandbergUniversitätsklinikum Hamburg-Eppendorf (UKE), Hamburg, Germany. magdalena.w.sandberg@gmail.com.ORCID http://orcid.org/0000-0003-1359-2474
Jakob BunkenborgAlphalyse A/S, Odense, Denmark.ORCID http://orcid.org/0000-0002-2908-9323
Stine ThyssenAlphalyse A/S, Odense, Denmark.
Martin VilladsenAlphalyse A/S, Odense, Denmark.
Thomas KofoedAlphalyse A/S, Odense, Denmark.
Universität Hamburg · DE

Funding

H2020 Marie Skłodowska-Curie Actions H2020-MSCA-ITN-2017
6 · The paper itself

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine and a white blood cell growth factor that has found usage as a therapeutic protein. During analysis of different fermentation batches of GM-CSF recombinantly expressed in E. coli, a covalent modification was identified on the protein by intact mass spectrometry. The modification gave a mass shift of + 70 Da and peptide mapping analysis demonstrated that it located to the protein N-terminus and lysine side chains. The chemical composition of C

Indexed as

Escherichia coliGranulocyte-Macrophage Colony-Stimulating FactorFermentationMass SpectrometryRecombinant ProteinsGranulocyte-Macrophage Colony-Stimulating FactorRecombinant ProteinsCrotonaldehydeE. coliGranulocyte-macrophage colony-stimulating factorPost-translation modificationRecombinant protein production

Identifiers

PMID34453584
PMCPMC9117350
OpenAlexW3193401466

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.