ArticleApplied microbiology and biotechnology2021
Purification, characterization, and mode of action of Paracin 54, a novel bacteriocin against Staphylococci.
Article in Applied microbiology and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 37 citations in OpenAlex.
- Pacin, a novel bacteriocin fromMicrobiology spectrum · 2026Article
- Synergistic anti-Helicobacter pylori efficacy of a molecularly identified Limosilactobacillus fermentum isolate in combination with multiple antibiotics.BMC microbiology · 2026Article
- Preliminary Purification and Partial Characterization of a Functional Bacteriocin of Lacticaseibacillus paracasei Zhang and Mining for its Gene Cluster.Probiotics and antimicrobial proteins · 2025Article
- Beyond Conventional Meat Preservation: Saddling the Control of Bacteriocin and Lactic Acid Bacteria for Clean Label and Functional Meat Products.Applied biochemistry and biotechnology · 2024Review
- Probiotic Properties ofFoods (Basel, Switzerland) · 2024Article
- Genome mining of Bacillus licheniformis MCC2514 for the identification of lasso peptide biosynthetic gene cluster and its characterization.Archives of microbiology · 2024Article
- Two Antimicrobial Peptides Derived fromMolecules (Basel, Switzerland) · 2023Article
- Purification, characterization, and mode of action of Sakacin ZFM225, a novel bacteriocin fromBiochemistry and biophysics reports · 2023Article
- A novel bacteriocin against multiple foodborne pathogens fromCurrent research in food science · 2023Article
- The Effect of Indole-3-Lactic Acid fromFoods (Basel, Switzerland) · 2022Article
- Antibacterial Effects of Bacteriocin PLNC8 againstFoods (Basel, Switzerland) · 2022Article
- Preventive and therapeutic effect ofFrontiers in nutrition · 2022Article
- Purification and characterization of bacteriocin produced by a strain ofFrontiers in microbiology · 2022Article
- A Novel Bacteriocin AgainstFrontiers in microbiology · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococci belong to conditionally pathogenic bacteria, and the pathogenicity of Staphylococcus aureus is the strongest among them. Enterotoxin produced by it can contaminate food and cause food poisoning. Bacteriocin is a kind of polypeptide with antibacterial activity synthesized by some bacteria during metabolism. In this study, we report on purification, characterization, and mode of action of the bacteriocin named Paracin 54, produced by Lactobacillus paracasei ZFM54. Paracin 54 was purified by precipitation with 80% ammonium sulfate, strong cation-exchange chromatography, G-25 gel column, and reversed-phase high-performance liquid chromatography (HPLC). The molecular weight of Paracin 54 (5718.1843 Da) was determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Paracin 54 showed broad-spectrum inhibitory activity. It had a strong inhibitory effect on Staphylococci with minimum inhibitory concentration values of 3.00-4.50 μg/mL. Paracin 54 was heat-stable and active only in acidic pH range (2-6). After treatment with proteases, the activity was lost. The results of mode of action showed Paracin 54 damaged the cell membrane and cell wall of Staphylococcus aureus, and then the cytoplasm leaked out, leading to death of the bacteria. These properties make Paracin 54 a promising candidate to prevent the growth of spoilage bacteria and control food poisoning caused by Staphylococci. KEY POINTS: • Paracin 54 was purified from Lactobacillus paracasei ZFM54 with good biochemical characteristics. • Paracin 54 had a strong effect against Staphylococci, making it a promising preservative to prevent the growth of Staphylococci in food. • The mode of action of Paracin 54 on Staphylococcus aureus was revealed.
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