Evidence map›Paper›PMID 34453537›Full record

ArticleThe Journal of infectious diseases2022

Flowcytometric and ImageStream Rna-Fish Gene Expression, Quantification and Phenotypic Characterization of Blood Sporozoites and Sporozoites From Human Malaria Species.

Camilla Luiza-Batista, Flore Nardella, Sabine Thiberge, Malika Serra-Hassoun, Marcelo U Ferreira, Artur Scherf, Sylvie Garcia

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Camilla Luiza-BatistaBiology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France.
Flore NardellaBiology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France.
Sabine ThibergeBiology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France.
Malika Serra-HassounBiology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France.
Marcelo U FerreiraDepartment of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Artur ScherfBiology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France.
Sylvie GarciaBiology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.

Indexed as

MalariaSporozoitesAnimalsGene ExpressionHumansPlasmodium falciparumPlasmodium vivaxProtozoan ProteinsRNAProtozoan ProteinsRNAflow analysesPlasmodiumRNA-FISH

Identifiers

PMID34453537
PMCPMC9071310

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.