ArticleScientific reports2021
A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 31 citations in OpenAlex.
- Finite element modeling of human Achilles tendon: impact of subtendon modeling and fiber direction.Biomechanics and modeling in mechanobiology · 2026Article
- Evolution of hierarchical phase-contrast tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study.Journal of synchrotron radiation · 2026Article
- Advancing three-dimensional tendon imaging using laboratory X-ray phase contrast techniques and refined sample preparation.Scientific reports · 2026Article
- Computed microtomographic imaging of revascularization during healing of Achilles tendon injury.Journal of anatomy · 2025Article
- Understanding the Structure-Function Relationship through 3D Imaging and Biomechanical Analysis: A Novel Methodological Approach Applied to Anterior Cruciate Ligaments.Biomimetics (Basel, Switzerland) · 2024Article
- In Situ Loading and Time-Resolved Synchrotron-Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof-of-Concept Study.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- High-Resolution Phase-Contrast Tomography on Human Collagenous Tissues: A Comprehensive Review.Tomography (Ann Arbor, Mich.) · 2023Review
- Heterotopic mineral deposits in intact rat Achilles tendons are characterized by a unique fiber-like structure.Journal of structural biology: X · 2023Article
- Significance of the broad non-bony attachments of the anterior cruciate ligament on the tibial side.Scientific reports · 2022Article
- A tomographic microscopy-compatible Langendorff system for the dynamic structural characterization of the cardiac cycle.Frontiers in cardiovascular medicine · 2022Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes). The image quality is assessed using four quantitative parameters. The results show that for studying tendon collagen fibers, conventional invasive sample preparations such as fixation and embedding are not necessary or advantageous. Instead, fresh frozen samples result in high-quality images that capture the complex 3D organization of tendon fibers in conditions as close as possible to natural. The comprehensive nature of this innovative study by SR-PhC-μCT breaks ground for future studies of soft complex biological tissue in 3D with high resolution in close to natural conditions, which could be further used for in situ characterization of how soft tissue responds to mechanical stimuli on a microscopic level.
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