ArticleScience advances2021
No ordinary proteins: Adsorption and molecular orientation of monoclonal antibodies.
Article in Science advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Precision nanomedicine for pulmonary diseases: from molecular targeting to clinical translation.Signal transduction and targeted therapy · 2026Review
- Predicting Agitation Stability of Monoclonal Antibodies during Developability Assessment.Molecular pharmaceutics · 2026Article
- Competitive adsorption of a monoclonal antibody and amphiphilic polymers to the air-water interface.European biophysics journal : EBJ · 2025Article
- Elucidating the role of carrier proteins in cytokine stabilization within double emulsion-based polymeric nanoparticles.Bioengineering & translational medicine · 2025Article
- pH-Dependent Conformational Plasticity of Monoclonal Antibodies at the SiOACS applied materials & interfaces · 2024Article
- Stability of Protein Pharmaceuticals: Recent Advances.Pharmaceutical research · 2024Review
- Adsorption of monoclonal antibody fragments at the water-oil interface: A coarse-grained molecular dynamics study.APL bioengineering · 2024Article
- Mechanistic Insights into the Adsorption of Monoclonal Antibodies at the Water/Vapor Interface.Molecular pharmaceutics · 2024Article
- The Adsorption of P2X2 Receptors Interacting with IgG Antibodies Revealed by Combined AFM Imaging and Mechanical Simulation.International journal of molecular sciences · 2023Article
- Antibodies Adsorbed to the Air-Water Interface Form Soft Glasses.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Dilatational and shear rheology of soluble and insoluble monolayers with a Langmuir trough.Journal of colloid and interface science · 2023Article
- Hydration in Deep Eutectic Solvents Induces Non-monotonic Changes in the Conformation and Stability of Proteins.Journal of the American Chemical Society · 2022Article
- Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces.Journal of visualized experiments : JoVE · 2022Article
- Differential Surface Adsorption Phenomena for Conventional and Novel Surfactants Correlates with Changes in Interfacial mAb Stabilization.Molecular pharmaceutics · 2022Article
- Versatile and Easily Designable Polyester-Laser Toner Interfaces for Site-Oriented Adsorption of Antibodies.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interaction of monoclonal antibodies (mAbs) with air/water interfaces plays a crucial role in their overall stability in solution. We aim to understand this behavior using pendant bubble measurements to track the dynamic tension reduction and x-ray reflectivity to obtain the electron density profiles (EDPs) at the surface. Native immunoglobulin G mAb is a rigid molecule with a flat, "Y" shape, and simulated EDPs are obtained by rotating a homology construct at the surface. Comparing simulations with experimental EDPs, we obtain surface orientation probability maps showing mAbs transition from flat-on Y-shape configurations to side-on or end-on configurations with increasing concentration. The modeling also shows the presence of β sheets at the surface. Overall, the experiments and the homology modeling elucidate the orientational phase space during different stages of adsorption of mAbs at the air/water interface. These finding will help define new strategies for the manufacture and storage of antibody-based therapeutics.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.