Evidence map›Paper›PMID 34452440›Full record

ArticleViruses2021

Comparison and Evaluation of Real-Time Taqman PCR for Detection and Quantification of Ebolavirus.

Yi Huang, Shuqi Xiao, Zhiming Yuan

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Yi HuangNational Biosafety Laboratory, Chinese Academy of Sciences, Wuhan 430020, China.ORCID 0000-0001-6585-5243
Shuqi XiaoWuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430020, China.
Zhiming YuanNational Biosafety Laboratory, Chinese Academy of Sciences, Wuhan 430020, China.ORCID 0000-0002-3234-9616

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Given that ebolavirus causes severe and frequently lethal disease, its rapid and accurate detection using available and validated methods is essential for controlling infection. Real-time reverse-transcription PCR (RT-PCR) has proven to be an invaluable tool for ebolaviruses diagnostics. Many assays with different targets have been developed, but they have not been externally compared or validated, and limits of detection are not uniformly reported. Here we compared and evaluated the sensitivity, reproducibility and specificity of 23 in-house assays under the same conditions. Our results showed that these assays were highly gene- and species- specific when evaluated using in vitro RNA transcripts and viral RNA, and the potential limits of detection were uniformly reported ranging from 10

Indexed as

EbolavirusHemorrhagic Fever, EbolaRNA, ViralHumansLimit of DetectionMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityRNA, Viralcomparisondetectionebolavirusevaluationquantificationreal-time TaqMan PCR

Identifiers

PMID34452440
PMCPMC8402893

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.