Evidence map›Paper›PMID 34452427›Full record

ArticleViruses2021

Comparative Characterization and Pathogenicity of a Novel Porcine Epidemic Diarrhea Virus (PEDV) with a Naturally Occurring Truncated ORF3 Gene Coinfected with PEDVs Possessing an Intact ORF3 Gene in Piglets.

Ying Lu, Weijian Huang, Lian Zhong, Yibin Qin, Xueting Liu, Chunjie Yang, Ruomu Wang, Xueli Su, Chen Du, Xue Mi and 6 more

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 2 institutions in 1 country.

Ying LuCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Weijian HuangCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Lian ZhongCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Yibin QinGuangxi Veterinary Research Institute, Nanning 530005, China.
Xueting LiuCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Chunjie YangCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Ruomu WangCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Xueli SuCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Chen DuCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Xue MiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Hejie WangCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Ying HeGuangxi Veterinary Research Institute, Nanning 530005, China.
Wu ZhaoGuangxi Veterinary Research Institute, Nanning 530005, China.
Ying ChenCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Zuzhang WeiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.ORCID 0000-0002-6317-2315
Kang OuyangCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Guangxi University · CNGuangxi Veterinary Research Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Coinfection caused by various genotypes of porcine epidemic diarrhea virus (PEDV) is a new disease situation. We previously reported the coexistence of PEDV strains containing different ORF3 genotypes in China. In this study, the PEDV strains 17GXCZ-1ORF3d and 17GXCZ-1ORF3c were isolated and plaque-purified from the same piglet, which had a natural large deletion at the 172-554 bp position of the ORF3 gene or possessed a complete ORF3 gene, respectively. Meanwhile, 17GXCZ-1ORF3d had >99% nt identity with 17GXCZ-1ORF3c in the 5'UTR, ORF1a/1b, S, E, M, N and 3'UTR regions but only demonstrated low nucleotide identities (80.5%) in the ORF3 gene. To elucidate the pathogenicity, 7-day-old piglets were infected. Piglets infected with these two PEDV strains exhibited severe clinical signs and shed the virus at the highest level within 96 hpi. Compared with the piglets inoculated with the 17GXCZ-1ORF3c strain, the piglets inoculated with the 17GXCZ-1ORF3d strain had higher mortality rates (75% vs. 50%), an earlier onset of clinical signs with a significantly higher diarrhea score, lower VH:CD ratios and a higher percentage of PEDV-positive enterocytes. This study is the first to report PEDV coinfections with different ORF3 genotypes, and a PEDV strain with a large deletion in the ORF3 gene might have the advantage of a potential genetic marker, which would be useful during vaccine development.

Indexed as

GenotypeAnimalsChlorocebus aethiopsCoinfectionCoronavirus InfectionsDiarrheaOpen Reading FramesPhylogenyPorcine epidemic diarrhea virusSwineSwine DiseasesVero CellsVirulencecoinfectionmolecular characteristicsORF3pathogenicityporcine epidemic diarrhea virusvirulence

Identifiers

PMID34452427
PMCPMC8402686
OpenAlexW3190099140

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.