ArticleViruses2021
Application of Oxford Nanopore Technology to Plant Virus Detection.
Article in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- From reads to results: comparing Oxford Nanopore to Illumina sequencing for citrus virus surveillance.BMC genomics · 2026Article
- Improving the diagnosis of cassava mosaic begomoviruses using Oxford Nanopore Technology sequencing.Scientific reports · 2025Article
- Managing Plant Viruses in Tissue-Cultured Apple and Grapevine: Strategies for Detection and Eradication.The plant pathology journal · 2025Review
- Nanopore Workflow for Grapevine Viroid Surveillance in Kazakhstan: Bypassing rRNA Depletion Through Non-Canonical Priming.Pathogens (Basel, Switzerland) · 2025Article
- Article
- Deciphering mixed infections by plant RNA virus and reconstructing complete genomes simultaneously present within-host.PloS one · 2025Article
- The Impact of Oxford Nanopore Technologies Based Methodologies on the Genome Sequencing and Assembly of Romanian Strains ofInsects · 2024Article
- High-Throughput Sequencing Methods for the Detection of Two Strawberry Viruses in Post-Entry Quarantine.Viruses · 2024Article
- Advancing pathogen surveillance by nanopore sequencing and genotype characterization of Acheta domesticus densovirus in mass-reared house crickets.Scientific reports · 2024Article
- Identification of Viruses InfectingInternational journal of molecular sciences · 2024Article
- The Oxford Nanopore MinION as a Versatile Technology for the Diagnosis and Characterization of Emerging Plant Viruses.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Application of Nanopore-Based Sequencing to Identify Virus Infections in Woody Plants.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Nanopore-Sequencing Metabarcoding for Identification of Phytopathogenic and Endophytic Fungi in Olive (Journal of fungi (Basel, Switzerland) · 2023Article
- Analysis of Wheat Virome in Korea Using Illumina and Oxford Nanopore Sequencing Platforms.Plants (Basel, Switzerland) · 2023Article
- Evaluation of sequencing and PCR-based methods for the quantification of the viral genome formula.Virus research · 2023Article
- New Virus Diagnostic Approaches to Ensuring the Ongoing Plant Biosecurity of Aotearoa New Zealand.Viruses · 2023Review
- NanoViromics: long-read sequencing of dsRNA for plant virus and viroid rapid detection.Frontiers in microbiology · 2023Article
- Brome mosaic virus detected in Kansas wheat co-infected with other common wheat viruses.Frontiers in plant science · 2023Article
- Article
- How do emerging long-read sequencing technologies function in transforming the plant pathology research landscape?Plant molecular biology · 2022Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adoption of Oxford Nanopore Technologies (ONT) sequencing as a tool in plant virology has been relatively slow despite its promise in more recent years to yield large quantities of long nucleotide sequences in real time without the need for prior amplification. The portability of the MinION and Flongle platforms combined with lowering costs and continued improvements in read accuracy make ONT an attractive method for both low- and high-scale virus diagnostics. Here, we provide a detailed step-by-step protocol using the ONT Flongle platform that we have developed for the routine application on a range of symptomatic post-entry quarantine and domestic surveillance plant samples. The aim of this methods paper is to highlight ONT's feasibility as a valuable component to the diagnostician's toolkit and to hopefully stimulate other laboratories towards the eventual goal of integrating high-throughput sequencing technologies as validated plant virus diagnostic methods in their own right.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.