Evidence map›Paper›PMID 34451396›Full record

ArticlePathogens (Basel, Switzerland)2021

Direct Amperometric Sensing of Fish Nodavirus RNA Using Gold Nanoparticle/DNA-Based Bioconjugates.

Nadia Chérif, Mohamed Zouari, Fatma Amdouni, Marwa Mefteh, Ayoub Ksouri, Balkiss Bouhaouala-Zahar, Noureddine Raouafi

Abstract read
In one paragraph

Article in Pathogens (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Nadia ChérifLaboratory of Aquaculture (LR 16INSTM03), National Institute of Marine Sciences and Technologies, 28 Rue de 2 Mars, Salamboo 1934, Tunisia.
Mohamed ZouariLaboratory of Analytical Chemistry and Electrochemistry (LR99ES15), Sensors and Biosensors Group, Tunis Faculty of Science, University of Tunis El Manar, Tunis 2092, Tunisia.
Fatma AmdouniLaboratory of Aquaculture (LR 16INSTM03), National Institute of Marine Sciences and Technologies, 28 Rue de 2 Mars, Salamboo 1934, Tunisia.
Marwa MeftehLaboratory of Analytical Chemistry and Electrochemistry (LR99ES15), Sensors and Biosensors Group, Tunis Faculty of Science, University of Tunis El Manar, Tunis 2092, Tunisia.
Ayoub KsouriLaboratory of Venoms and Therapeutic Molecules (LR16IPT08), 1" Place Pasteur, BP74, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis 1002, Tunisia.
Balkiss Bouhaouala-ZaharLaboratory of Venoms and Therapeutic Molecules (LR16IPT08), 1" Place Pasteur, BP74, Pasteur Institute of Tunis, University of Tunis El Manar, Tunis 1002, Tunisia.ORCID 0000-0003-3147-245X
Noureddine RaouafiLaboratory of Analytical Chemistry and Electrochemistry (LR99ES15), Sensors and Biosensors Group, Tunis Faculty of Science, University of Tunis El Manar, Tunis 2092, Tunisia.ORCID 0000-0001-8938-8221

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe the design of a simple and highly sensitive electrochemical bioanalytical method enabling the direct detection of a conserved RNA region within the capsid protein gene of a fish nodavirus, making use of nanostructured disposable electrodes. To achieve this goal, we select a conserved region within the nodavirus RNA2 segment to design a DNA probe that is tethered to the surface of nanostructured disposable screen-printed electrodes. In a proof-of-principle test, a synthetic RNA sequence is detected based on competitive hybridization between two oligonucleotides (biotinylated reporter DNA and target RNA) complimentary to a thiolated DNA capture probe. The method is further validated using extracted RNA samples obtained from healthy carrier

Indexed as

amperometrybetanodavirusdiagnosisfishnanosensorsRT-PCR

Identifiers

PMID34451396
PMCPMC8398327

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.