ArticleChemosphere2022
Critical new insights into the binding of poly- and perfluoroalkyl substances (PFAS) to albumin protein.
Article in Chemosphere, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- Preserved and Accessible: Quantifying PFAS in Formalin-Fixed and Paraffin-Embedded Tissues for Retrospective Exposure and Dose Assessment.Environmental science & technology · 2026Article
- Review
- Toward Comprehensive In Vitro Evaluation of Serum Albumin Binding of Per- and Polyfluoroalkyl Substances.Journal of xenobiotics · 2026Review
- The Impact of Forever Chemicals on Protein Structure and Function.International journal of molecular sciences · 2026Review
- NAMs for closing knowledge gaps in assessment of respiratory uptake - a PARC project.Frontiers in toxicology · 2026Article
- Toxicokinetics and Perfluorooctanesulfonic Acid-Induced Liver Protein Expression Are Markedly Altered in Mice Lacking Albumin.Chemical research in toxicology · 2025Article
- Fluorinated Zirconium-Based Metal-Organic Frameworks as Novel Sorbents to Improve the Efficacy of Hemodialysis Treatment.Small science · 2025Article
- Research Progress in Current and Emerging Issues of PFASs' Global Impact: Long-Term Health Effects and Governance of Food Systems.Foods (Basel, Switzerland) · 2025Review
- Underlying Mechanisms of Chromatographic H/D, H/F,Metabolites · 2025Review
- An in vitro and machine learning framework for quantifying serum albumin binding of per- and polyfluoroalkyl substances.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Investigating the quantitative toxicological relationship between PFAS alkyl fluorine structure and exposure levels leading to changes in blood-based clinical markers in rats.Journal of toxicology and environmental health. Part A · 2025Article
- PFAS-Biomolecule Interactions: Case Study Using Asclepios Nodes and Automated Workflows in KNIME for Drug Discovery and Toxicology.Methods in molecular biology (Clifton, N.J.) · 2025Article
- In silico prediction of the interaction of legacy and novel per- and poly-fluoroalkyl substances (PFAS) with selected human transporters and of their possible accumulation in the human body.Archives of toxicology · 2024Article
- Unbound Fractions of PFAS in Human and Rodent Tissues: Rat Liver a Suitable Proxy for Evaluating Emerging PFAS?Environmental science & technology · 2024Article
- Evaluation of 14 PFAS for permeability and organic anion transporter interactions: Implications for renal clearance in humans.Chemosphere · 2024Article
- Species Difference? Bovine, Trout, and Human Plasma Protein Binding of Per- and Polyfluoroalkyl Substances.Environmental science & technology · 2024Article
- Maternal PFOS exposure affects offspring development in Nrf2-dependent and independent ways in zebrafish (Danio rerio).Aquatic toxicology (Amsterdam, Netherlands) · 2024Article
- An Atomic and Molecular Insight into How PFOA Reduces α-Helicity, Compromises Substrate Binding, and Creates Binding Pockets in a Model Globular Protein.Journal of the American Chemical Society · 2024Article
- Quantitative cross-species comparison of serum albumin binding of per- and polyfluoroalkyl substances from five structural classes.Toxicological sciences : an official journal of the Society of Toxicology · 2024Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
With an increasing number of health-related impacts of per- and polyfluoroalkyl substances (PFAS) being reported, there is a pressing need to understand PFAS transport within both the human body and the environment. As proteins can serve as a primary transport mechanism for PFAS, understanding PFAS binding to proteins is essential for predictive physiological models where accurate values of protein binding constants are vital. In this work we present a critical analysis of three common models for analyzing PFAS binding to bovine serum albumin (BSA) based on fluorescence quenching: the Stern-Volmer model, the modified Stern-Volmer model, and the Hill equation. The PFAS examined include perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorodecanoic acid (PFDA), perfluorobutanesulfonic acid (PFBS), perfluorohexanesulfonic acid (PFHxS), perfluorooctanesulfonic acid (PFOS), and the replacement compound 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy)propanoate (HFPO-DA or GenX). While all three models capture the general effects of hydrophobicity and steric limitations to PFAS binding, the Hill equation highlighted a unique relationship between binding cooperativity and the number of fluorinated carbons, with PFOA exhibiting the greatest binding cooperativity. The significance of steric limitations was confirmed by comparing results obtained by fluorescence quenching, which is an indirect method based on specific binding, to those obtained by equilibrium dialysis where PFAS binding directly correlated with traditional measures of hydrophobicity. Finally, the binding constants were correlated with PFAS physicochemical properties where van der Waals volume best described the steric limitations observed by fluorescence quenching.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.