ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2021
DMRT1 gene disruption alone induces incomplete gonad feminization in chicken.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Review
- Efficient and Safe Knockout ofVeterinary sciences · 2026Article
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- Manipulating primordial germ cells from chicken and other bird species.The Journal of reproduction and development · 2026Review
- Single-nucleus transcriptional and chromatin accessible profiles reveal critical cell types and molecular architecture underlying chicken sex determination.Journal of advanced research · 2025Article
- Genome Assembly of a Living Fossil, the Atlantic Horseshoe Crab Limulus polyphemus, Reveals Lineage-Specific Whole-Genome Duplications, Transposable Element-Based Centromeres, and a ZW Sex Chromosome System.Molecular biology and evolution · 2025Article
- Article
- Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells.Frontiers in genome editing · 2024Article
- Review
- RNA sequencing analysis of sexual dimorphism in Japanese quail.Frontiers in veterinary science · 2024Article
- Dosage compensation of Z sex chromosome genes in avian fibroblast cells.Genome biology · 2023Article
- Female-Biased Expression ofAnimals : an open access journal from MDPI · 2023Article
- Preliminary Study on Expression and Function of the Chicken W Chromosome GeneInternational journal of molecular sciences · 2023Article
- Overview of Avian Sex Reversal.International journal of molecular sciences · 2023Review
- Review
- Review
- Review
- Dissecting chicken germ cell dynamics by combining a germ cell tracing transgenic chicken model with single-cell RNA sequencing.Computational and structural biotechnology journal · 2022Article
- Article
- Becoming female: Ovarian differentiation from an evolutionary perspective.Frontiers in cell and developmental biology · 2022Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Compared with the well-described XY sex determination system in mammals, the avian ZW sex determination system is poorly understood. Knockdown and overexpression studies identified doublesex and mab-3-related transcription factor 1 (DMRT1) as the testis-determining gene in chicken. However, the detailed effects of DMRT1 gene disruption from embryonic to adult development are not clear. Herein, we have generated DMRT1-disrupted chickens using the clustered regularly interspaced short palindromic repeats-associated protein 9 system, followed by an analysis of physiological, hormonal, and molecular changes in the genome-modified chickens. In the early stages of male chicken development, disruption of DMRT1 induced gonad feminization with extensive physiological and molecular changes; however, functional feminine reproductivity could not be implemented with disturbed hormone synthesis. Subsequent RNA-sequencing analysis of the DMRT1-disrupted chicken gonads revealed gene networks, including several novel genes linearly and non-linearly associated with DMRT1, which are involved in gonad feminization. By comparing the gonads of wild type with the genome-modified chickens, a set of genes were identified that is involved in the ZW sex determination system independent of DMRT1. Our results extend beyond the Z-dosage hypothesis to provide further information about the avian ZW sex determination system and epigenetic effects of gonad feminization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.