Evidence map›Paper›PMID 34448066›Full record

ArticleMolecular biology reports2021

Iranian Strawberry crinkle cytorhabdovirus variation assessed using its movement protein (P3) gene.

Mohammad Hajizadeh, Nasrin Ghaderi Zandan

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Mohammad HajizadehDepartment of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran. m.hajizadeh@uok.ac.ir.ORCID http://orcid.org/0000-0002-0774-3523
Nasrin Ghaderi ZandanDepartment of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
University of Kurdistan · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundStrawberry crinkle virus (SCV) is a member of the genus Cytorhabdovirus, family Rhabdovirida, and order Mononegavirales. SCV affects the production of various strawberry cultivars. In this study we investigated the genetic diversity of SCV in strawberry fields based on P3 (movement protein) gene. METHODS AND

resultsThe samples were collected from strawberry fields in the Kurdistan Province, Iran. P3 gene from 20 SCV isolates, representing 18 nucleic acid haplotypes, is composed of 729 nucleotides, encoding a protein with 243 amino acids. SCV-P3 sequences shared 98.77%-99.86% nucleotide and 97.5%-100% amino acid sequence identity. Phylogenetic analyses of the new P3 sequences with two previously published SCV-P3 sequences from the Czech Republic showed that there are two major phylogroups (I and II) and three minor phylogroups in the body of the phylogeny, I-1, I-2, II-1. Comparisons of P3 gene sequences revealed a mutational bias, with more differences being transitions than transversions. The ratio of non-synonymous/synonymous nucleotide changes was < 1, indicating that SCV-P3 gene is under predominantly negative selection.

conclusionsPhylogenetic and sequence identity analyses showed that SCV isolates from Iran are closely related and have not diverged more than 2% based on P3 gene despite geographical separation and strawberry cultivar. This is the first report of the genetic diversity of SCV worldwide.

Indexed as

Genes, ViralGenetic VariationAmino Acid SequenceBase SequenceCodonData AnalysisFragariaGeographyIranLikelihood FunctionsPhylogenyPlant Viral Movement ProteinsRhabdoviridaeCodonPlant Viral Movement ProteinsKurdistan ProvinceMovement proteinPhylogenetic treePositive selection

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PMID34448066
OpenAlexW3193304132

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