ReviewNature methods2021
The power of imaging to understand extracellular vesicle biology in vivo.
Open access · bronzeAbstract readReview
In one paragraph
Review in Nature methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 192 papers.
0numbers the graph read from it
0cells of the map it votes in
192citing papers in PubMed
19.6field-weighted citation impact, top 1% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
192 citing papers in PubMed, 318 citations in OpenAlex.
- Towards a systematic understanding of the diverse subtypes, biogenesis and functions of large extracellular vesicles.Nature reviews. Molecular cell biology · 2026Review
- Extracellular vesicles: A new therapeutic drug for nerve injury repair.Neural regeneration research · 2026Article
- A Novel Reporter Mouse for Astrocyte-Derived Extracellular Vesicles Reveals Enrichment of Cargo at Neuronal Mitochondria.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Review
- Maternal obesity programs offspring metabolic dysfunction via small extracellular vesicle-mediated epigenetic remodeling.Nature communications · 2026Article
- Human Platelet-Derived Extracellular Vesicles Are Internalized by Human Induced Pluripotent Stem Cell-Derived Neurons Under Control and Hypoxic Conditions.Journal of extracellular biology · 2026Article
- EV-SPEC: Decoding the Spatial Proteomic Signature of the Extracellular Vesicle Corona.Journal of extracellular biology · 2026Article
- Extracellular vesicle cargo dynamics in the bone marrow microenvironment: from hematopoietic homeostasis to malignant transformation.Extracellular vesicle · 2026Article
- A Nanoparticle-Integrated Complete Manufacturing Pipeline of Chemically Engineered Exosomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Glycosylation of Extracellular Vesicles: Analytical and Translational Insights into Biomarker Discovery and Regenerative Medicine.International journal of molecular sciences · 2026Review
- Quality Evaluation Considerations for Stem Cell-Derived Extracellular Vesicles-Based Therapeutic Products in China.Journal of extracellular vesicles · 2026Review
- Exercise and Skeletal Muscle-Derived Extracellular Vesicles: Their Cargo, Release, and Role in Metabolic Regulation.Journal of extracellular biology · 2026Review
- No Dye Fits All: Illuminating the Challenges of Fluorescent Extracellular Vesicle Labeling.Journal of extracellular vesicles · 2026Article
- TLR7 induces autophagy in non-small cell lung cancer tumor cells and influences anti-tumors responses in patients.Translational lung cancer research · 2026Article
- A novel reporter mouse for astrocyte-derived extracellular vesicles reveals trafficking of cargo to neuronal mitochondria.bioRxiv : the preprint server for biology · 2026Article
- Application of extracellular vesicles in the CRISPR-based diagnosis and treatment: possibilities and challenges.Journal of biological engineering · 2026Review
- Endogenous Labelling of Extracellular Vesicles and Image Capture of Their Interactions With Acceptor Cells.Chembiochem : a European journal of chemical biology · 2026Article
- Nanomedicine novel strategies: deciphering the EV-metabolic axis as a natural nanocarrier network in lung cancer progression and cachexia.Journal of nanobiotechnology · 2026Review
- Circulating extracellular vesicle microRNAs mediate immune modulation of social behavior in male mice.Nature communications · 2026Article
- Contribution of tumor-derived extracellular vesicles in the establishment of the pre-metastatic niche: lessons learned from past experimentations and future directions.Clinical & experimental metastasis · 2026Review
132 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
29 authors at 20 institutions in 13 countries.
Frederik J VerweijUniversité de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Paris, France. frederikverweij@gmail.com.ORCID http://orcid.org/0000-0001-8879-1174
Leonora Balaj *Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0003-0931-9715
Chantal M Boulanger *Université de Paris, PARCC, INSERM, Paris, France.
David R F Carter *Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, UK.
Ewoud B Compeer *Kennedy Institute of Rheumatology, NDORMS, University of Oxford, Oxford, UK.ORCID http://orcid.org/0000-0002-3050-7633
Gisela D'Angelo *Institut Curie, PSL Research University, CNRS, UMR144 Cell Biology and Cancer, Paris, France.ORCID http://orcid.org/0000-0003-3150-2241
Samir El Andaloussi *Evox Therapeutics Limited, Oxford Science Park, Oxford, UK.
Jacky G Goetz *INSERM UMR_S1109, Tumor Biomechanics Lab, Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Equipe Labellisée Ligue contre le Cancer, Strasbourg, France.ORCID http://orcid.org/0000-0003-2842-8116
Julia Christina Gross *Health and Medical University, Potsdam, Germany.ORCID http://orcid.org/0000-0002-8939-5664
Vincent Hyenne *INSERM UMR_S1109, Tumor Biomechanics Lab, Université de Strasbourg, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Equipe Labellisée Ligue contre le Cancer, Strasbourg, France.
Eva-Maria Krämer-Albers *Johannes Gutenberg-Universität Mainz, Institute of Developmental Biology and Neurobiology, Mainz, Germany.ORCID http://orcid.org/0000-0001-7994-1185
Charles P Lai *Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, Taiwan.ORCID http://orcid.org/0000-0001-8050-7060
Xavier Loyer *Université de Paris, PARCC, INSERM, Paris, France.
Alex Marki *La Jolla Institute for Allergy and Immunology, La Jolla, CA, USA.
Stefan Momma *Institute of Neurology (Edinger Institute), Goethe-University, Frankfurt am Main, Germany.ORCID http://orcid.org/0000-0001-7816-5145
Esther N M Nolte-'t Hoen *Department of Biomolecular Health Sciences, Faculty of veterinary medicine, Utrecht University, Utrecht, the Netherlands.
D Michiel Pegtel *Amsterdam UMC, Vrije Universiteit Amsterdam, Pathology, Cancer Center Amsterdam, Amsterdam, the Netherlands.
Hector Peinado *Microenvironment and Metastasis Laboratory, Molecular Oncology Programme, Spanish National Cancer Research Center (CNIO), Madrid, Spain.ORCID http://orcid.org/0000-0002-4256-3413
Graça Raposo *Institut Curie, PSL Research University, CNRS, UMR144 Cell Biology and Cancer, Paris, France.
Kirsi Rilla *University of Eastern Finland, Institute of Biomedicine, Kuopio, Finland.ORCID http://orcid.org/0000-0002-7862-5727
Hidetoshi Tahara *Department of Cellular and Molecular Biology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.ORCID http://orcid.org/0000-0003-3054-4175
Clotilde Théry *Institut Curie, PSL Research University, INSERM U932, Immunity and Cancer, Paris, France.ORCID http://orcid.org/0000-0001-8294-6884
Martin E van Royen *Erasmus MC, Department of Pathology, Rotterdam, the Netherlands.
Roosmarijn E Vandenbroucke *VIB Center for Inflammation Research and Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.ORCID http://orcid.org/0000-0002-8327-620X
Ann M Wehman *Department of Biological Sciences, University of Denver, Denver, CO, USA.ORCID http://orcid.org/0000-0001-9826-4132
Kenneth Witwer *Department of Molecular and Comparative Pathobiology and Neurology and the Richman Family Precision Medicine Center of Excellence in Alzheimer's Disease, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Zhiwei Wu *Center for Public Health Research, Medical School, Nanjing University, Nanjing, China.
Richard Wubbolts *Department of Biomolecular Health Sciences, Faculty of veterinary medicine, Utrecht University, Utrecht, the Netherlands.ORCID http://orcid.org/0000-0001-8661-7594
Guillaume van NielUniversité de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Paris, France. guillaume.van-niel@inserm.fr.ORCID http://orcid.org/0000-0002-8651-9705
Inserm · FRCentre National de la Recherche Scientifique · FRUtrecht University · NLAmsterdam University Medical Centers · NLErasmus MC · NLGhent University · BEGoethe University Frankfurt · DEHarvard University · USHiroshima University · JPInstitute of Atomic and Molecular Sciences, Academia Sinica · TWJohannes Gutenberg University Mainz · DEJohns Hopkins University · USKarolinska Institutet · SELaboratoire de Biologie et Modélisation de la Cellule · FRLa Jolla Institute for Immunology · USNanjing Medical University · CNOxford Brookes University · GBSpanish National Cancer Research Centre · ESUniversity of Denver · USUniversity of Eastern Finland · FI
Funding
The role of microvesicles in giloma virotherapyP01CA069246 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI CHIOCCA, E. ANTONIO · 1996 to 2021
$30.9MNext-generation extracellular vesicle biologics to target central nervous system and peripheral reservoirs of HIVR01AI144997 · NIAID · JOHNS HOPKINS UNIVERSITY · PI RAMRATNAM, BHARAT, WITWER, KENNETH W · 2019 to 2023
$4.0MLiquid Biopsy for treatment stratification of IDH1.R132H and EGFRvIII mutant gliomasU01CA230697 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI CARTER, BOB S, SKOG, JOHAN · 2018 to 2022
$4.0MExtracellular Vesicle and Extracellular RNA Biomarkers of HIV-1 Central Nervous System Pathogenesis and Cigarette UseR01DA047807 · NIDA · JOHNS HOPKINS UNIVERSITY · PI WITWER, KENNETH W · 2018 to 2022
$3.7MImaging and Liquid Biopsy for Glioma Diagnosis and Treatment MonitoringR01CA239078 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI BALAJ, LEONORA, LEE, HAKHO · 2020 to 2024
$3.1MStandardized Molecular Analyses of Glioma EVsR01CA237500 · NCI · MASSACHUSETTS GENERAL HOSPITAL · PI CARTER, BOB S, LEE, HAKHO · 2020 to 2024
$3.0MNovel Approaches to Capture, Sorting, and Characterization of CNS-Origin Extracellular VesiclesR33MH118164 · NIMH · JOHNS HOPKINS UNIVERSITY · PI MACHAIRAKI, VASILIKI, WITWER, KENNETH W · 2020 to 2022
$2.2MNovel Separation Methods for exRNA Carriers: Extracellular Vesicles, Lipoprotein Particles, and Protein AggregatesUG3CA241694 · NCI · JOHNS HOPKINS UNIVERSITY · PI WITWER, KENNETH W · 2019 to 2020
$941kBiotechnology and Biological Sciences Research Council BB/P006205/1Cancer Research UK A28052NCI NIH HHS P01 CA069246NCI NIH HHS R01 CA237500NCI NIH HHS R01 CA239078NCI NIH HHS U01 CA230697NCI NIH HHS UG3 CA241694NIAID NIH HHS R01 AI144997NIDA NIH HHS R01 DA047807NIMH NIH HHS R33 MH118164
Abstract
Extracellular vesicles (EVs) are nano-sized lipid bilayer vesicles released by virtually every cell type. EVs have diverse biological activities, ranging from roles in development and homeostasis to cancer progression, which has spurred the development of EVs as disease biomarkers and drug nanovehicles. Owing to the small size of EVs, however, most studies have relied on isolation and biochemical analysis of bulk EVs separated from biofluids. Although informative, these approaches do not capture the dynamics of EV release, biodistribution, and other contributions to pathophysiology. Recent advances in live and high-resolution microscopy techniques, combined with innovative EV labeling strategies and reporter systems, provide new tools to study EVs in vivo in their physiological environment and at the single-vesicle level. Here we critically review the latest advances and challenges in EV imaging, and identify urgent, outstanding questions in our quest to unravel EV biology and therapeutic applications.
Indexed as
Extracellular VesiclesAnimalsColoring AgentsEpitopesFluorescent DyesHumansMicroscopyColoring AgentsEpitopesFluorescent Dyes
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.