Evidence map›Paper›PMID 34446532›Full record

ArticleRNA (New York, N.Y.)2021

Synthesis of modified nucleotide polymers by the poly(U) polymerase Cid1: application to direct RNA sequencing on nanopores.

Jenny Mai Vo, Logan Mulroney, Jen Quick-Cleveland, Miten Jain, Mark Akeson, Manuel Ares

Erratum issuedOpen access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jenny Mai Vo *Department of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California 95064, USA.ORCID 0000-0002-8250-4709
Logan Mulroney *Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California 95064, USA.ORCID 0000-0002-0534-0165
Jen Quick-ClevelandDepartment of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California 95064, USA.ORCID 0000-0002-4070-8842
Miten JainDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California 95064, USA.ORCID 0000-0002-4571-3982
Mark AkesonDepartment of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, California 95064, USA.ORCID 0000-0001-9392-8075
Manuel AresDepartment of Molecular, Cell, and Developmental Biology, University of California Santa Cruz, Santa Cruz, California 95064, USA.ORCID 0000-0002-2552-9168
University of California, Santa Cruz · US

Funding

STRUCTURE/FUNCTION OF YEAST SMALL NUCLEAR RNPSR01GM040478 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI ARES, MANUEL · 1988 to 2021
$10.8M
A Unified Nanopore Platform for Direct Sequencing of Individual Full Length RNA Strands Bearing Modified NucleotidesR01HG010053 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI AKESON, MARK A · 2018 to 2021
$3.8M
Structure, regulation, and evolution of the splicing machineryR35GM145266 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI Manuel Ares · 2022 to 2026
$2.5M
NHGRI NIH HHS R01 HG010053NIGMS NIH HHS R01 GM040478NIGMS NIH HHS R35 GM145266
6 · The paper itself

Abstract

Understanding transcriptomes requires documenting the structures, modifications, and abundances of RNAs as well as their proximity to other molecules. The methods that make this possible depend critically on enzymes (including mutant derivatives) that act on nucleic acids for capturing and sequencing RNA. We tested two 3' nucleotidyl transferases,

Indexed as

NanoporesNucleotidesNucleotidyltransferasesPolymersPolynucleotide AdenylyltransferaseSaccharomyces cerevisiaeSchizosaccharomycesSchizosaccharomyces pombe ProteinsSequence Analysis, RNACid1 protein, S pombeNucleotidesNucleotidyltransferasesPolymersPolynucleotide AdenylyltransferaseSchizosaccharomyces pombe Proteins

Identifiers

PMID34446532
PMCPMC8594468
OpenAlexW3195224616

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.