ArticleMicrobial cell factories2021
Improving citric acid production of an industrial Aspergillus niger CGMCC 10142: identification and overexpression of a high-affinity glucose transporter with different promoters.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 30 citations in OpenAlex.
- Fermentation-based oxalic acid production aimed at sustainable critical metal recovery from electronic waste.Applied microbiology and biotechnology · 2026Article
- Transporter Engineering for Enhancing Citric Acid Production inJournal of fungi (Basel, Switzerland) · 2026Article
- Enhancing lipase activity in Aspergillus niger through CRISPR/Cas9-mediated protease gene knockout and fermentation optimization.Biotechnology letters · 2025Article
- Promoter engineering with programmable upstream activating sequences in Aspergillus Niger cell factory.Microbial cell factories · 2025Article
- Bioleaching for critical metal recovery from bauxite residue- unlocking waste valorization.Frontiers in bioengineering and biotechnology · 2025Review
- An overview of key industrial product citric acid production by Aspergillus niger and its application.Journal of industrial microbiology & biotechnology · 2024Review
- Beyond Penicillin: The Potential of Filamentous Fungi for Drug Discovery in the Age of Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2023Review
- Engineering a Phosphoketolase Pathway to Supplement Cytosolic Acetyl-CoA inJournal of fungi (Basel, Switzerland) · 2023Article
- Unlocking the magic in mycelium: Using synthetic biology to optimize filamentous fungi for biomanufacturing and sustainability.Materials today. Bio · 2023Article
- Sustainable citric acid production from COFrontiers in microbiology · 2022Article
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8 authors at 1 institution in 1 country.
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Abstract
backgroundGlucose transporters play an important role in the fermentation of citric acid. In this study, a high-affinity glucose transporter (HGT1) was identified and overexpressed in the industrial strain A. niger CGMCC 10142. HGT1-overexpressing strains using the PglaA and Paox1 promoters were constructed to verify the glucose transporter functions.
resultAs hypothesized, the HGT1-overexpressing strains showed higher citric acid production and lower residual sugar contents. The best-performing strain A. niger 20-15 exhibited a reduction of the total sugar content and residual reducing sugars by 16.5 and 44.7%, while the final citric acid production was significantly increased to 174.1 g/L, representing a 7.3% increase compared to A. niger CGMCC 10142. Measurement of the mRNA expression levels of relevant genes at different time-points during the fermentation indicated that in addition to HGT1, citrate synthase and glucokinase were also expressed at higher levels in the overexpression strains.
conclusionThe results indicate that HGT1 overexpression resolved the metabolic bottleneck caused by insufficient sugar transport and thereby improved the sugar utilization rate. This study demonstrates the usefulness of the high-affinity glucose transporter HGT1 for improving the citric acid fermentation process of Aspergillus niger CGMCC 10142.
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