ArticleAnimal bioscience2022
Cysteine improves boar sperm quality via glutathione biosynthesis during the liquid storage.
Article in Animal bioscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Optimization of Semen Extender for Mongolian Sheep by Integrating Metabolomics and Sperm Quality Analysis at 17 °C with Antioxidant Supplementation.Animals : an open access journal from MDPI · 2026Article
- Cryopreservation of biological materials: applications and economic perspectives.In vitro cellular & developmental biology. Animal · 2026Review
- Mechanisms of seminal plasma synthesis and actions on sperm function for advancing reproductive technologies.The Journal of reproduction and development · 2026Review
- Oleic Acid Improves Goat Sperm Quality by Enhancing the MBOAT2/ACSL3 Pathway to Attenuate Ferroptosis.Animals : an open access journal from MDPI · 2025Article
- Role of Redox-Induced Protein Modifications in Spermatozoa in Health and Disease.Antioxidants (Basel, Switzerland) · 2025Review
- Effect of idebenone supplementation in a semen extender on boar spermatozoa quality during liquid storage.Veterinary world · 2025Article
- Mitochonic acid 5 increases boar sperm quality by mitigating mitochondrial dysfunction.Frontiers in cell and developmental biology · 2025Article
- Effects of Varying Light Durations on Sperm Quality in Rams.Animals : an open access journal from MDPI · 2024Article
- Metabolomic signature of sperm in men with obesity-associated asthenozoospermia.Journal of assisted reproduction and genetics · 2024Article
- β-Nicotinamide mononucleotide improves chilled ram sperm quality in vitro by reducing oxidative stress damage.Animal bioscience · 2024Article
- Mitochonic Acid 5 Increases Ram Sperm Quality by Improving Mitochondrial Function during Storage at 4 °C.Animals : an open access journal from MDPI · 2024Article
- Fertility after photodynamic inactivation of bacteria in extended boar semen.Frontiers in microbiology · 2024Article
- Oxido-Reduction Potential as a Method to Determine Oxidative Stress in Semen Samples.International journal of molecular sciences · 2023Article
- Beneficial Effect of Proline Supplementation on Goat Spermatozoa Quality during Cryopreservation.Animals : an open access journal from MDPI · 2022Article
- Carboxylated ε-Poly-L-Lysine Supplementation of the Freezing Extender Improves the Post-Thawing Boar Sperm Quality.Animals : an open access journal from MDPI · 2022Article
- The effects of cysteine and L-carnitine on the DNA integrity of post-thaw sperm of frozen buck semen.Iranian journal of veterinary research · 2022Article
- Caffeic Acid Phenethyl Ester (CAPE) Improves Boar Sperm Quality and Antioxidant Capacity in Liquid Preservation (17°C) Linked to AMPK Activity Maintenance.Frontiers in veterinary science · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveSperm is particularly susceptible to reactive oxygen species (ROS) stress. Glutathione (GSH) is an endogenous antioxidant that regulates sperm redox homeostasis. However, it is not clear whether boar sperm could utilize cysteine for synthesis GSH to protect sperm quality from ROS damage. Therefore, the present study was undertaken to elucidate the mechanism of how cysteine is involved in protecting boar sperm quality during liquid storage.
methodsSperm motility, membrane integrity, lipid peroxidation, 4-hydroxyIlonenal (4-HNE) modifications, mitochondrial membrane potential, as well as the levels of ROS, GSH, and, ATP were evaluated. Moreover, the enzymes (GCLC: glutamate cysteine ligase; GSS: glutathione synthetase) that are involved in glutathione synthesis from cysteine precursor were detected by western blotting.
resultsCompared to the control, addition of 1.25 mM cysteine to the liquid storage significantly increased boar sperm progressive motility, straight-line velocity, curvilinear velocity, beat-cross frequency, membrane integrity, mitochondrial membrane potential, ATP level, acrosome integrity, activities of superoxide dismutase and catalase, and GSH level, while reducing the ROS level, lipid peroxidation and 4-HNE modifications. It was also observed that the GCLC and GSS were expressed in boar sperm. Interestingly, when we used menadione to induce sperm with ROS stress, the menadione associated damages were observed to be reduced by the cysteine supplementation. Moreover, compared to the cysteine treatment, the γ-glutamylcysteine synthetase (γ-GCS) activity, GSH level, mitochondrial membrane potential, ATP level, membrane integrity and progressive motility in boar sperm were decreased by supplementing with an inhibitor of GSH synthesis, buthionine sulfoximine.
conclusionThese data suggest that boar sperm could biosynthesize the GSH from cysteine in vitro. Therefore, during storage, addition of cysteine improves boar sperm quality via enhancing the GSH synthesis to resist ROS stress.
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