Evidence map›Paper›PMID 34445554›Full record

ArticleInternational journal of molecular sciences2021

Endogenous Enzymatic Activity in Dentin Treated with a Chitosan Primer.

Tatjana Maravić, Eugenia Baena, Claudia Mazzitelli, Uroš Josić, Edoardo Mancuso, Vittorio Checchi, Luigi Generali, Laura Ceballos, Lorenzo Breschi, Annalisa Mazzoni

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Tatjana MaravićDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, Via San Vitale 59, 40125 Bologna, Italy.
Eugenia BaenaArea of Stomatology, Health Sciences Faculty, King Juan Carlos University, Avda. de Atenas, 28922 Alcorcón, Spain.ORCID 0000-0002-9092-8675
Claudia MazzitelliDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, Via San Vitale 59, 40125 Bologna, Italy.
Uroš JosićDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, Via San Vitale 59, 40125 Bologna, Italy.
Edoardo MancusoDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, Via San Vitale 59, 40125 Bologna, Italy.
Vittorio ChecchiDepartment of Surgery, Medicine, Dentistry and Morphological Sciences with Transplant Surgery, Oncology and Regenerative Medicine Relevance, University of Modena & Reggio Emilia, 41124 Modena, Italy.ORCID 0000-0002-3053-5562
Luigi GeneraliDepartment of Surgery, Medicine, Dentistry and Morphological Sciences with Transplant Surgery, Oncology and Regenerative Medicine Relevance, University of Modena & Reggio Emilia, 41124 Modena, Italy.ORCID 0000-0003-3764-7046
Laura CeballosArea of Stomatology, Health Sciences Faculty, King Juan Carlos University, Avda. de Atenas, 28922 Alcorcón, Spain.
Lorenzo BreschiDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, Via San Vitale 59, 40125 Bologna, Italy.ORCID 0000-0001-7621-226X
Annalisa MazzoniDepartment of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna-Alma Mater Studiorum, Via San Vitale 59, 40125 Bologna, Italy.
University of Bologna · ITUniversidad Rey Juan Carlos · ESUniversity of Modena and Reggio Emilia · IT

Funding

European Federation of Conservative Dentistry EFCD Scientific Foundation scholarship for Eugenia Baena
6 · The paper itself

Abstract

The aim of this study was to evaluate the effect of different concentrations of chitosan polymer on dentinal enzymatic activity by means of gelatin and in situ zymography. Human dentin was frozen and ground in a miller. Dentin powder aliquots were demineralized with phosphoric acid and treated with three different concentrations of lyophilized chitosan polymer (1, 0.5 and 0.1 wt%) dissolved in distilled water. Dentin proteins were extracted from each experimental group and electrophoresed under non-reducing conditions in 10% SDS-PAGE containing fluorescein-labeled gelatin. After 48 h in the incubation buffer at 37 °C, proteolytic activity was registered under long-wave UV light scanner and quantified by using Image J software. Furthermore, additional teeth (

Indexed as

Chelating AgentsChitosanDentinHumansMaterials TestingMatrix MetalloproteinasesChelating AgentsChitosanMatrix Metalloproteinaseschitosancollagen cross-linkerdentinmatrix metalloproteinaseszymography

Identifiers

PMID34445554
PMCPMC8396363
OpenAlexW3195032269

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.