ArticleInternational journal of molecular sciences2021
A New Glycosyltransferase Enzyme from Family 91, UGT91P3, Is Responsible for the Final Glucosylation Step of Crocins in Saffron (
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 53 citations in OpenAlex.
- Natural CCD2 Variants and RNA Interference for Boosting Crocin Biosynthesis in Tomato.Biology · 2025Article
- Montbretia flowers as a source of bioactive crocins: Biotechnology tools and delivery systems.Biotechnology reports (Amsterdam, Netherlands) · 2025Article
- Water-soluble carotenoid: focused on natural carotenoid crocin.Food science and biotechnology · 2025Review
- Effects of supplementary light treatment on saffron: integrated physiological, metabolomic, and transcriptome analyses.BMC plant biology · 2024Article
- The biosynthesis of asiaticoside and madecassoside reveals tandem duplication-directed evolution of glycoside glycosyltransferases in the Apiales.Plant communications · 2024Article
- Co-regulatory network analysis of the main secondary metabolite (SM) biosynthesis in Crocus sativus L.Scientific reports · 2024Article
- N-terminal truncated phospholipase A1 accessory protein PlaS from Serratia marcescens alleviates inhibitory on host cell growth and enhances PlaA1 enzymatic activity.Bioresources and bioprocessing · 2024Article
- Metabolomic and transcriptomic analyses of yellow-flowered crocuses to infer alternative sources of saffron metabolites.BMC plant biology · 2024Article
- Article
- Research progress on the pharmacological activity, biosynthetic pathways, and biosynthesis of crocins.Beilstein journal of organic chemistry · 2024Review
- Research Progress in Heterologous Crocin Production.Marine drugs · 2023Review
- Production of Saffron Apocarotenoids inMetabolites · 2023Article
- Terpenoid Transport in Plants: How Far from the Final Picture?Plants (Basel, Switzerland) · 2023Review
- Molecular Cloning, Expression, and Functional Analysis of Glycosyltransferase (TbUGGT) Gene fromMolecules (Basel, Switzerland) · 2022Article
- Understanding saffron biology using omics- and bioinformatics tools: stepping towards a better Crocus phenome.Molecular biology reports · 2022Article
- Plant carotenoids: recent advances and future perspectives.Molecular horticulture · 2022Review
- Fortification and bioaccessibility of saffron apocarotenoids in potato tubers.Frontiers in nutrition · 2022Article
- Changes of Crocin and Other Crocetin Glycosides in Saffron Through Cooking Models, and Discovery of Rare Crocetin Glycosides in the Yellow Flowers ofFrontiers in nutrition · 2022Article
- Metabolic Engineering of Crocin Biosynthesis inFrontiers in plant science · 2022Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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Abstract
Crocetin is an apocarotenoid formed from the oxidative cleavage of zeaxanthin, by the carotenoid cleavage enzymes CCD2 (in Crocus species) and specific CCD4 enzymes in Buddleja davidii and Gardenia jasminoides. Crocetin accumulates in the stigma of saffron in the form of glucosides and crocins, which contain one to five glucose molecules. Crocetin glycosylation was hypothesized to involve at least two enzymes from superfamily 1 UDP-sugar dependent glycosyltransferases. One of them, UGT74AD1, produces crocins with one and two glucose molecules, which are substrates for a second UGT, which could belong to the UGT79, 91, or 94 families. An in silico search of Crocus transcriptomes revealed six candidate UGT genes from family 91. The transcript profiles of one of them, UGT91P3, matched the metabolite profile of crocin accumulation, and were co-expressed with UGT74AD1. In addition, both UGTs interact in a two-hybrid assay. Recombinant UGT91P3 produced mostly crocins with four and five glucose molecules in vitro, and in a combined transient expression assay with CCD2 and UGT74AD1 enzymes in Nicotiana benthamiana. These results suggest a role of UGT91P3 in the biosynthesis of highly glucosylated crocins in saffron, and that it represents the last missing gene in crocins biosynthesis.
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