ArticleInternational journal of molecular sciences2021
Image-Based Method to Quantify Decellularization of Tissue Sections.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- In Vitro Evaluation of Escherichia coli and Staphylococcus aureus Translocation in 3D Printed Material.Journal of biomedical materials research. Part A · 2026Article
- Extracellular stiffness regulates cell fate determination and drives the emergence of evolutionary novelty in the teleost heart.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Acellular Extracellular Matrix Scaffolds in Regenerative Medicine: Advances in Decellularization and Clinical Applications.Journal of functional biomaterials · 2025Review
- Determination of residual DNA in decellularised aortas- towards fluorescence-based quantification of DNA purified by various methods.Molecular biology reports · 2025Article
- Synthesis of Metal-Modified Nanocellulose as a Biofilm Analogue for Biofilm Mimicry in Biomedical and Environmental Applications.Biopolymers · 2025Article
- Effects of aging on the biomechanical properties of the lung extracellular matrix: dependence on tissular stretch.Frontiers in cell and developmental biology · 2024Article
- Article
- Multi-Step Extracellular Matrix Remodelling and Stiffening in the Development of Idiopathic Pulmonary Fibrosis.International journal of molecular sciences · 2023Article
- Engineering Dynamic 3D Models of Lung.Advances in experimental medicine and biology · 2023Article
- A Fast and Efficient Decellularization Method for Tissue Slices.Bio-protocol · 2022Article
- Novel Decellularization Method for Tissue Slices.Frontiers in bioengineering and biotechnology · 2022Article
- Recent Advances in Liver Engineering With Decellularized Scaffold.Frontiers in bioengineering and biotechnology · 2022Review
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Authors and funding
6 authors.
Funding
Abstract
Tissue decellularization is typically assessed through absorbance-based DNA quantification after tissue digestion. This method has several disadvantages, namely its destructive nature and inadequacy in experimental situations where tissue is scarce. Here, we present an image processing algorithm for quantitative analysis of DNA content in (de)cellularized tissues as a faster, simpler and more comprehensive alternative. Our method uses local entropy measurements of a phase contrast image to create a mask, which is then applied to corresponding nuclei labelled (UV) images to extract average fluorescence intensities as an estimate of DNA content. The method can be used on native or decellularized tissue to quantify DNA content, thus allowing quantitative assessment of decellularization procedures. We confirm that our new method yields results in line with those obtained using the standard DNA quantification method and that it is successful for both lung and heart tissues. We are also able to accurately obtain a timeline of decreasing DNA content with increased incubation time with a decellularizing agent. Finally, the identified masks can also be applied to additional fluorescence images of immunostained proteins such as collagen or elastin, thus allowing further image-based tissue characterization.
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