Evidence map›Paper›PMID 34442869›Full record

ArticleMicroorganisms2021

Rational Design for Enhanced Acyltransferase Activity in Water Catalyzed by the

Amanda Staudt, Henrik Terholsen, Jasmin Kaur, Henrik Müller, Simon P Godehard, Ivaldo Itabaiana, Ivana C R Leal, Uwe T Bornscheuer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Rational design of enzyme activity and enantioselectivity.Frontiers in bioengineering and biotechnology · 2023
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Amanda StaudtDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, 17487 Greifswald, Germany.
Henrik TerholsenDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, 17487 Greifswald, Germany.ORCID 0000-0001-9625-8177
Jasmin KaurDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, 17487 Greifswald, Germany.
Henrik MüllerDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, 17487 Greifswald, Germany.
Simon P GodehardDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, 17487 Greifswald, Germany.
Ivaldo ItabaianaLaboratory of Technological Biochemistry and Biocatalysis, Department of Biochemical Engineering, School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro 21941-909, Brazil.ORCID 0000-0003-0725-0881
Ivana C R LealLaboratory of Natural Products and Biological Assays, Department of Natural Products and Food, Faculty of Pharmacy, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Uwe T BornscheuerDepartment of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, 17487 Greifswald, Germany.ORCID 0000-0003-0685-2696
Universität Greifswald · DEUniversidade Federal do Rio de Janeiro · BR

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Finance Code 001
6 · The paper itself

Abstract

Biocatalytic transesterification is commonly carried out employing lipases in anhydrous organic solvents since hydrolases usually prefer hydrolysis over acyl transfer in bulk water. However, some promiscuous acyltransferases can catalyze acylation in an aqueous solution. In this study, a rational design was performed to enhance the acyltransferase selectivity and substrate scope of the

Indexed as

acyl transferacyltransferasebiocatalysismonoterpene acylationPestEprotein engineeringtransesterification

Identifiers

PMID34442869
PMCPMC8402108
OpenAlexW3195495531

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.