Evidence map›Paper›PMID 34442750›Full record

ArticleMicroorganisms2021

Distribution of SARS-CoV-2 Lineages in the Czech Republic, Analysis of Data from the First Year of the Pandemic.

Petr Klempt, Ondřej Brzoň, Martin Kašný, Kateřina Kvapilová, Petr Hubáček, Aleš Briksi, Matěj Bezdíček, Vladimira Koudeláková, Martina Lengerová, Marian Hajdúch and 5 more

Abstract read
In one paragraph

Article in Microorganisms, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Petr KlemptInstitute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.
Ondřej BrzoňInstitute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.
Martin KašnýInstitute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.
Kateřina KvapilováInstitute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.
Petr HubáčekDepartment of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, V Úvalu 84, 150 06 Prague, Czech Republic.
Aleš BriksiDepartment of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, V Úvalu 84, 150 06 Prague, Czech Republic.
Matěj BezdíčekCenter of Molecular Biology and Genetics, Department of Internal Medicine-Haematology and Oncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Černopolní 212/9, 625 00 Brno, Czech Republic.ORCID 0000-0002-5833-8325
Vladimira KoudelákováLaboratory of Experimental Medicine, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Hněvotínská 5, 77 515 Olomouc, Czech Republic.
Martina LengerováCenter of Molecular Biology and Genetics, Department of Internal Medicine-Haematology and Oncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Černopolní 212/9, 625 00 Brno, Czech Republic.
Marian HajdúchLaboratory of Experimental Medicine, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Hněvotínská 5, 77 515 Olomouc, Czech Republic.
Pavel DřevínekDepartment of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, V Úvalu 84, 150 06 Prague, Czech Republic.ORCID 0000-0002-8994-5240
Šárka PospíšilováCenter of Molecular Biology and Genetics, Department of Internal Medicine-Haematology and Oncology, University Hospital Brno and Faculty of Medicine, Masaryk University, Černopolní 212/9, 625 00 Brno, Czech Republic.
Eva KriegováDepartment of Immunology, Faculty of Medicine and Dentistry, Palacký University Olomouc and University Hospital, Hněvotínská 3, 775 15 Olomouc, Czech Republic.ORCID 0000-0002-8969-4197
Milan MacekDepartment of Biology and Medical Genetics, 2nd Faculty of Medicine-Charles University and Motol University Hospital, V Úvalu 84, 150 05 Prague, Czech Republic.
Petr KvapilInstitute of Applied Biotechnologies, Služeb 3056/4, 108 00 Prague, Czech Republic.

Funding

MSMT.cz CZ.02.1.01/0.0/0.0/16_026/000844, LM2018132MZCR.cz 00064203/6003RVO FNBr, 65269705 Ministry of Health, Czech Republic
6 · The paper itself

Abstract

In the Czech Republic, the current pandemic led to over 1.67 million SARS-CoV-2- positive cases since the recording of the first case on 1 March 2020. SARS-CoV-2 genome analysis is an important tool for effective real-time quantitative PCR (RT-qPCR) diagnostics, epidemiology monitoring, as well as vaccination strategy. To date, there is no comprehensive report on the distribution of SARS-CoV-2 genome variants in either the Czech Republic, including Central and Eastern Europe in general, during the first year of pandemic. In this study, we have analysed a representative cohort of SARS-CoV-2 genomes from 229 nasopharyngeal swabs of COVID-19 positive patients collected between March 2020 and February 2021 using validated reference-based sequencing workflow. We document the changing frequency of dominant variants of SARS-CoV-2 (from B.1 -> B.1.1.266 -> B.1.258 -> B.1.1.7) throughout the first year of the pandemic and list specific variants that could impact the diagnostic efficiency RT-qPCR assays. Moreover, our reference-based workflow provided evidence of superinfection in several samples, which may have contributed to one of the highest per capita numbers of COVID-19 cases and deaths during the first year of the pandemic in the Czech Republic.

Indexed as

massively parallel sequencingmetagenomicsphylogenySARS-CoV-2variants

Identifiers

PMID34442750
PMCPMC8397935

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.