Evidence map›Paper›PMID 34442721›Full record

ArticleMicroorganisms2021

Culture-Independent and Culture-Dependent Characterization of the Black Soldier Fly Gut Microbiome Reveals a Large Proportion of Culturable Bacteria with Potential for Industrial Applications.

Dorothee Tegtmeier, Sabine Hurka, Sanja Mihajlovic, Maren Bodenschatz, Stephanie Schlimbach, Andreas Vilcinskas

Abstract read
In one paragraph

Article in Microorganisms, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Selection andACS agricultural science & technology · 2026
    Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Article
  11. Review
  12. Investigating the Microbial Dynamics ofFoods (Basel, Switzerland) · 2025
    Article
  13. Article
  14. Article
  15. The integral role of the Black Soldier fly,Frontiers in microbiomes · 2025
    Review
  16. Article
  17. Turning trash into treasure:Applied and environmental microbiology · 2024
    Article
  18. Black Soldier Fly (Animals : an open access journal from MDPI · 2024
    Article
  19. Gut microorganisms ofmSystems · 2024
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Dorothee TegtmeierBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 35392 Giessen, Germany.ORCID 0000-0003-1814-9041
Sabine HurkaInstitute for Insect Biotechnology, Justus Liebig University, 35392 Giessen, Germany.ORCID 0000-0003-2083-5198
Sanja MihajlovicBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 35392 Giessen, Germany.
Maren BodenschatzBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 35392 Giessen, Germany.
Stephanie SchlimbachBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 35392 Giessen, Germany.
Andreas VilcinskasBranch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), 35392 Giessen, Germany.ORCID 0000-0001-8276-4968

Funding

Bundesministerium für Bildung und Forschung FKZ 031A533Hessisches Ministerium für Wissenschaft und Kunst
6 · The paper itself

Abstract

Black soldier fly larvae (BSFL) are fast-growing, resilient insects that can break down a variety of organic substrates and convert them into valuable proteins and lipids for applications in the feed industry. Decomposition is mediated by an abundant and versatile gut microbiome, which has been studied for more than a decade. However, little is known about the phylogeny, properties and functions of bacterial isolates from the BSFL gut. We therefore characterized the BSFL gut microbiome in detail, evaluating bacterial diversity by culture-dependent methods and amplicon sequencing of the 16S rRNA gene. Redundant strains were identified by genomic fingerprinting and 105 non-redundant isolates were then tested for their ability to inhibit pathogens. We cultivated representatives of 26 genera, covering 47% of the families and 33% of the genera detected by amplicon sequencing. Among these isolates, we found several representatives of the most abundant genera:

Indexed as

16S rRNA geneamplicon sequencingblack soldier flycore microbiomeculturable microbiomegenotypingpathogen inhibition

Identifiers

PMID34442721
PMCPMC8398798

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.