Evidence map›Paper›PMID 34442518›Full record

ArticleMicromachines2021

Sternal Bone Marrow Harvesting and Culturing Techniques from Patients Undergoing Cardiac Surgery.

Jimmy J H Kang, Sabin J Bozso, Ryaan El-Andari, Michael C Moon, Darren H Freed, Jayan Nagendran, Jeevan Nagendran

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jimmy J H KangDivision of Cardiac Surgery, University of Alberta, Edmonton, AB T6G 2B7, Canada.
Sabin J BozsoDivision of Cardiac Surgery, University of Alberta, Edmonton, AB T6G 2B7, Canada.
Ryaan El-AndariFaculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G 2B7, Canada.ORCID 0000-0001-7146-4337
Michael C MoonDivision of Cardiac Surgery, University of Alberta, Edmonton, AB T6G 2B7, Canada.ORCID 0000-0001-6617-8336
Darren H FreedDivision of Cardiac Surgery, University of Alberta, Edmonton, AB T6G 2B7, Canada.ORCID 0000-0002-6239-1444
Jayan NagendranDivision of Cardiac Surgery, University of Alberta, Edmonton, AB T6G 2B7, Canada.ORCID 0000-0001-5827-9823
Jeevan NagendranDivision of Cardiac Surgery, University of Alberta, Edmonton, AB T6G 2B7, Canada.ORCID 0000-0002-2050-6717
University of Alberta · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMesenchymal stromal cells (MSCs) are the most prominent cell type used in clinical regenerative medicine and stem cell research. MSCs are commonly harvested from bone marrow that has been aspirated from patients' iliac crest. However, the ethical challenges of finding consenting patients and obtaining fresh autologous cells via invasive extraction methods remain to be barriers to MSC research.

methodsTechniques of harvesting sternal bone marrow, isolating and culturing MSCs, MSC surface phenotyping, and MSC differentiation are described. Samples from 50 patients undergoing a sternotomy were collected, and the time taken to reach 80% confluency and cell count at the second splitting of MSC were measured.

resultsMSC isolated from the sternal bone marrow of patients undergoing cardiac surgery demonstrated successful MSC surface phenotyping and MSC differentiation. The mean cell count at the time of the second split was 1,628,025, and the mean time taken to reach the second split was 24.8 days.

conclusionHerein, we describe the first reported technique of harvesting sternal bone marrow from patients already undergoing open-chest cardiac surgery to reduce the invasiveness of bone marrow harvesting, as well as the methods of isolating, culturing, and identifying MSCs for the clinical application of constructing autologous MSC-derived biomaterials.

Indexed as

bone marrow harvestisolation and culturingmesenchymal stromal cellsstemtissue engineering

Identifiers

PMID34442518
PMCPMC8397946
OpenAlexW3192519425

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.