Evidence map›Paper›PMID 34442362›Full record

ArticleJournal of personalized medicine2021

Dose-Dependent Effect of Cordycepin on Viability, Proliferation, Cell Cycle, and Migration in Dental Pulp Stem Cells.

Nezar Boreak, Ahmed Alkahtani, Khalid Alzahrani, Amani Hassan Kenani, Wafa Hussain Faqehi, Hadeel Hussain Faqehi, Raghad Essa Ageeli, Wafa Nasser Moafa, Hosam Ali Baeshen, Shilpa Bhandi and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of personalized medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Effect ofMolecules (Basel, Switzerland) · 2022
    Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Nezar BoreakDepartment of Restorative Dental Sciences, College of Dentistry, Restorative Dental Sciences Jazan University, Jazan 45142, Saudi Arabia.ORCID 0000-0001-9017-9224
Ahmed AlkahtaniDepartment of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11362, Saudi Arabia.
Khalid AlzahraniDepartment of Clinical Laboratories Sciences, College of Applied Medical Sciences, Taif University, Taif 21944, Saudi Arabia.ORCID 0000-0002-6688-0106
Amani Hassan KenaniCollege of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
Wafa Hussain FaqehiCollege of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
Hadeel Hussain FaqehiCollege of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
Raghad Essa AgeeliCollege of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
Wafa Nasser MoafaCollege of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
Hosam Ali BaeshenDepartment of Orthodontics, College of Dentistry, King Abdulaziz University, Jeddah 11451, Saudi Arabia.
Shilpa BhandiDepartment of Restorative Dental Sciences, College of Dentistry, Restorative Dental Sciences Jazan University, Jazan 45142, Saudi Arabia.
Zohaib KhurshidDepartment of Prosthodontics and Dental Implantology, College of Dentistry, King Faisal University, Al-Ahsa 31982, Saudi Arabia.ORCID 0000-0001-7998-7335
Vikrant R PatilBiogenre Private Limited, Pune 412105, India.ORCID 0000-0003-0017-5807
Luca TestarelliDepartment of Oral and Maxillofacial Sciences, Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0003-3904-3000
Shankargouda PatilDivision of Oral Pathology, Department of Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry Jazan University, Jazan 45142, Saudi Arabia.ORCID 0000-0001-7246-5497

Funding

Taif University TURSP-2020/128
6 · The paper itself

Abstract

objectiveTo examine the effect of Cordycepin on the viability, proliferation, and migratory properties of dental pulp-derived mesenchymal stem cells. MATERIALS AND

methodsThe pulp was derived from human premolar teeth extracted for orthodontic purposes after obtaining informed consent. The samples were transferred to the laboratory for processing. DPSCs were expanded and characterized using flow cytometry and differentiation to the bone, adipose, and cartilage cells was examined. MTT Assay was performed using various concentrations of Cordycepin. The growth curve was plotted for 13 days. Cell cycle analysis was performed by flow cytometry. Migratory ability was assessed by wound healing assay. ROS generation was detected by flow cytometry. Gene expression was quantified by RT-qPCR. Statistical analysis was performed.

resultsDPSCs expressed characteristic MSC-specific markers and trilineage differentiation. Cordycepin at lower concentrations did not affect the viability of DPSCs. The growth curve of cells showed a dose-dependent increase in cell numbers till the maximum dose. DPSCs treated with 2.5 µM Cordycepin was found to have a reduced G1 phase cell percentage. DPSCs treated with 2.5 µM and 5 µM Cordycepin showed a significant decrease in G2 phase cells. No significant difference was observed for S phase cells. Cordycepin treatment affected the migratory ability in DPSCs in a concentration-dependent manner.

conclusionCordycepin can be used at therapeutic doses to maintain stem cells.

Indexed as

cell cycleCordycepindental pulp stem cellsgrowth curve

Identifiers

PMID34442362
PMCPMC8398271

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.