ArticleFoods (Basel, Switzerland)2021
Development of an RNA Extraction Protocol for Norovirus from Raw Oysters and Detection by qRT-PCR and Droplet-Digital RT-PCR.
Article in Foods (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed.
- Correlation Between Infectivity and qRT-PCR Values for Murine Norovirus Recovered from Frozen Berries.Food and environmental virology · 2025Article
- Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus.Food and environmental virology · 2025Article
- Public health implications of multidrugresistant and methicillinresistant Staphylococcus aureus in retail oysters.Scientific reports · 2025Article
- Genomic characterization of noroviruses from an outbreak associated with oysters.Microbiology spectrum · 2025Article
- Public health concern of antimicrobial resistance and virulence determinants in E. coli isolates from oysters in Egypt.Scientific reports · 2024Article
- Occurrence of carbapenem-resistant hypervirulent Klebsiella pneumoniae in oysters in Egypt: a significant public health issue.Annals of clinical microbiology and antimicrobials · 2024Article
- Spatial Distribution and Enrichment Dynamics of Foodborne Norovirus in Oyster Tissues.Foods (Basel, Switzerland) · 2023Article
- Amplicon-Based High-Throughput Sequencing Method for Genotypic Characterization of Norovirus in Oysters.Applied and environmental microbiology · 2023Article
- Research progress on detection techniques for point-of-care testing of foodborne pathogens.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Foodborne viruses such as norovirus and hepatitis A virus cause frequent outbreaks associated with the consumption of raw or undercooked oysters. Viral particles are bioaccumulated in the oyster's digestive glands, making RNA extraction and RT-PCR detection difficult due to the complex nature of the food matrix and the presence of RT-PCR inhibitors. Herein, we have developed a viral RNA extraction protocol from raw oysters using murine norovirus (MNV) as a surrogate for human noroviruses. The method combines lysis in Tri-Reagent reagent, followed by RNA extraction using Direct-Zol purification columns and lithium chloride precipitation. Viral load quantification was performed by both qRT-PCR and droplet-digital RT-PCR. We have demonstrated that this method can efficiently remove RT-PCR inhibitors, and is sensitive enough to reliably detect viral contamination at 25 PFU/0.2 g. We have also compared the efficiency of this method with the ISO 15216-1:2017 method and Method E developed by Quang and colleagues, and observed significantly higher efficiency compared with the ISO 15216-1 method and comparable efficiency with Method E, with less steps, and shorter hands-on time.
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Registered trials
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