Evidence map›Paper›PMID 34441486›Full record

ArticleFoods (Basel, Switzerland)2021

Non-Specific Binding and Cross-Reaction of ELISA: A Case Study of Porcine Hemoglobin Detection.

Xingyi Jiang, Meng Wu, Jonathan Albo, Qinchun Rao

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Double-antigen sandwich ELISA based on chimeric antigens for detection of antibodies to Trypanosoma cruzi in human sera - A phase II study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026
    Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Alpha-N-acetylgalactosaminidase in cancer: diagnostic applications and related treatment strategies.International journal of physiology, pathophysiology and pharmacology · 2025
    Review
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Frontiers in cellular and infection microbiology · 2023
    Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Xingyi JiangDepartment of Nutrition and Integrative Physiology, Florida State University, Tallahassee, FL 32306, USA.
Meng WuInstitute of Biology, Hebei Academy of Sciences, Shijiazhuang 050081, China.
Jonathan AlboMeinig School of Biomedical Engineering, Cornell University, Ithaca, NY 14853, USA.
Qinchun RaoDepartment of Nutrition and Integrative Physiology, Florida State University, Tallahassee, FL 32306, USA.ORCID 0000-0002-6853-5381

Funding

National Institute of Food and Agriculture 2017-70001-25984
6 · The paper itself

Abstract

Different types of enzyme-linked immunosorbent assays (ELISA) have been widely used to control food safety and quality. To develop an accurate and reproducible ELISA, false immunodetection results caused by non-specific binding (NSB) and cross-reaction must be prevented. During the case study of sandwich ELISA development for the detection of porcine hemoglobin (P

Indexed as

antibodycross-reactionELISAnon-specific bindingvalidation

Identifiers

PMID34441486
PMCPMC8394222

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.