Unlocking

William A C Gendron, Jeffrey D Rubin, Michael J Hansen, Rebecca A Nace, Brandon W Simone, Stephen C Ekker, Michael A Barry

Abstract read

PubMed Full text at PMC Publisher

In one paragraph

Article in Genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Mapping FirstbioRxiv : the preprint server for biology · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

William A C GendronVirology and Gene Therapy Graduate Program, Mayo Clinic Graduate School of Biomedical Sciences, Rochester, MN 55905, USA.

Jeffrey D RubinVirology and Gene Therapy Graduate Program, Mayo Clinic Graduate School of Biomedical Sciences, Rochester, MN 55905, USA.ORCID 0000-0001-9168-4361

Michael J HansenVirology and Gene Therapy Graduate Program, Mayo Clinic Graduate School of Biomedical Sciences, Rochester, MN 55905, USA.

Rebecca A NaceVirology and Gene Therapy Graduate Program, Mayo Clinic Graduate School of Biomedical Sciences, Rochester, MN 55905, USA.

Brandon W SimoneDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.ORCID 0000-0003-3907-3015

Stephen C EkkerDepartment of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.

Michael A BarryDepartment of Medicine, Division of Infectious Diseases, Mayo Clinic, Rochester, MN 55905, USA.ORCID 0000-0002-8568-5664

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development of CRISPR-associated proteins, such as Cas9, has led to increased accessibility and ease of use in genome editing. However, additional tools are needed to quantify and identify successful genome editing events in living animals. We developed a method to rapidly quantify and monitor gene editing activity non-invasively in living animals that also facilitates confocal microscopy and nucleotide level analyses. Here we report a new CRISPR "fingerprinting" approach to activating luciferase and fluorescent proteins in mice as a function of gene editing. This system is based on experience with our prior cre recombinase (cre)-detector system and is designed for Cas editors able to target

Indexed as

Gene EditingAnimalsCRISPR-Cas SystemsGreen Fluorescent ProteinsIntegrasesLuciferasesMiceCre recombinaseGreen Fluorescent ProteinsIntegrasesLuciferasesCreCRISPRErCas12afluorescent reportergene editingin vivoloxPluciferase reporternaked DNA injectionSaCas9

Identifiers

PMID34440379

DOI10.3390/genes12081204

PMCPMC8394901

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.