Evidence map›Paper›PMID 34440312›Full record

ArticleGenes2021

A Rapid and Cost-Effective Identification of Invertebrate Pests at the Borders Using MinION Sequencing of DNA Barcodes.

Shamila Weerakoon Abeynayake, Sonia Fiorito, Adrian Dinsdale, Mark Whattam, Bill Crowe, Kate Sparks, Paul Richard Campbell, Cherie Gambley

Abstract read
In one paragraph

Article in Genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shamila Weerakoon AbeynayakePlant Innovation Centre, Plant Import Operations, Biosecurity Plant Division, Department of Agriculture, Water and the Environment (DAWE), Canberra, ACT 2601, Australia.
Sonia FioritoPlant Innovation Centre, Plant Import Operations, Biosecurity Plant Division, Department of Agriculture, Water and the Environment (DAWE), Canberra, ACT 2601, Australia.
Adrian DinsdalePlant Innovation Centre, Plant Import Operations, Biosecurity Plant Division, Department of Agriculture, Water and the Environment (DAWE), Canberra, ACT 2601, Australia.
Mark WhattamPlant Innovation Centre, Plant Import Operations, Biosecurity Plant Division, Department of Agriculture, Water and the Environment (DAWE), Canberra, ACT 2601, Australia.
Bill CroweOperational Science and Surveillance, Science and Surveillance Group, Biosecurity Plant Division, Department of Agriculture, Water and the Environment (DAWE), Canberra, ACT 2601, Australia.
Kate SparksOperational Science and Surveillance, Science and Surveillance Group, Biosecurity Plant Division, Department of Agriculture, Water and the Environment (DAWE), Canberra, ACT 2601, Australia.
Paul Richard CampbellMicrobiology and Entomology, Biosciences, Department of Agriculture and Fisheries, Queensland Government (DAF, QLD), Brisbane, QLD 4001, Australia.
Cherie GambleyMicrobiology and Entomology, Biosciences, Department of Agriculture and Fisheries, Queensland Government (DAF, QLD), Brisbane, QLD 4001, Australia.ORCID 0000-0003-2283-8720

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The rapid and accurate identification of invertebrate pests detected at the border is a challenging task. Current diagnostic methods used at the borders are mainly based on time consuming visual and microscopic examinations. Here, we demonstrate a rapid in-house workflow for DNA extraction, PCR amplification of the barcode region of the mitochondrial cytochrome oxidase subunit I (

Indexed as

Cost-Benefit AnalysisAnimalsDNA Barcoding, TaxonomicInsectaInvertebratesSequence Analysis, DNAbiosecuritycytochrome c oxidase subunit Iinvertebrate pestsMinION sequencing

Identifiers

PMID34440312
PMCPMC8392835

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.