ArticleGenes2021
Gene Expression Analysis Provides New Insights into the Mechanism of Intramuscular Fat Formation in Japanese Black Cattle.
Article in Genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Super-Enhancer-Associated Gene 3-Hydroxybutyrate Dehydrogenase 1 Increases Intramuscular Fat Deposition of Yaks (Foods (Basel, Switzerland) · 2026Article
- Comparative Morphological and Proteomic Characterization of Adipose Tissues from Different Anatomical Sites in Yili Horses.Biology · 2026Article
- Article
- Integrated proteomics and metabolomics elucidate HSD17B12 regulation of intramuscular fat deposition for enhanced beef quality.Food chemistry. Molecular sciences · 2025Article
- Single-Nucleus RNA Sequencing Reveals Muscle-Region-Specific Differences in Fibro-Adipogenic Progenitors Driving Intramuscular Fat Accumulation.Metabolites · 2025Article
- Article
- Vitamin D₃ inhibits proliferation and the early stage of adipogenic differentiation but promotes gene expression and lipid accumulation during the late stage of adipogenic differentiation in intramuscular adipose cells of Korean native cattle.Journal of animal science · 2025Article
- Article
- Genome-Wide Association Study of Conformation Traits in Brazilian Holstein Cattle.Animals : an open access journal from MDPI · 2024Article
- A single-cell atlas of bovine skeletal muscle reveals mechanisms regulating intramuscular adipogenesis and fibrogenesis.Journal of cachexia, sarcopenia and muscle · 2023Article
- Accurate haplotype construction and detection of selection signatures enabled by high quality pig genome sequences.Nature communications · 2023Article
- Genome-Wide Association Analysis of Muscle pH in Texel Sheep × Altay Sheep FAnimals : an open access journal from MDPI · 2023Article
- Diversity in Cell Morphology, Composition, and Function among Adipose Depots in River Buffaloes.International journal of molecular sciences · 2023Article
- Candidate Genes and Gene Networks Change with Age in Japanese Black Cattle by Blood Transcriptome Analysis.Genes · 2023Article
- Integrated Analysis of Long Non-Coding RNA and mRNA to Reveal Putative Candidate Genes Associated with Backfat Quality in Beijing Black Pig.Foods (Basel, Switzerland) · 2022Article
- Polo-Like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5.International journal of molecular sciences · 2022Article
- Application of Mass Spectrometry for Determining the Geographic Production Area of Wagyu Beef.Metabolites · 2022Article
- Production of Hydroxy Fatty Acids, Precursors of γ-Hexalactone, Contributes to the Characteristic Sweet Aroma of Beef.Metabolites · 2022Article
- Transcriptome Analysis of Bovine Rumen Tissue in Three Developmental Stages.Frontiers in genetics · 2022Article
- Genome-Wide Selection Signatures and Human-Mediated Introgression Events inFrontiers in genetics · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Japanese Black cattle (Japanese Wagyu) have a unique phenotype in which ectopic intramuscular fat accumulates in skeletal muscle, producing finely marbled beef. However, the mechanism of intramuscular fat formation in Japanese Black cattle remains unclear. To investigate the key genes involved in intramuscular fat accumulation, we comprehensively analyzed mRNA levels in subcutaneous and intramuscular fat tissues using RNA sequence (RNA-seq) analysis, which detected 27,606 genes. We identified eight key genes, namely carboxypeptidase E, tenascin C, transgelin, collagen type IV alpha 5 (COL4A5), cysteine and glycine-rich protein 2, PDZ, and LIM domain 3, phosphatase 1 regulatory inhibitor subunit 14A, and regulator of calcineurin 2. These genes were highly and specifically expressed in intramuscular fat tissue. Immunohistochemical analysis revealed a collagen network, including COL4A5, in the basement membrane around the intramuscular fat tissue. Moreover, pathway analysis revealed that, in intramuscular fat tissue, differentially expressed genes are related to cell adhesion, proliferation, and cancer pathways. Furthermore, pathway analysis showed that the transforming growth factor-β (TGF-β) and small GTPases regulators RASGRP3, ARHGEF26, ARHGAP10, ARHGAP24, and DLC were upregulated in intramuscular fat. Our study suggests that these genes are involved in intramuscular fat formation in Japanese Black cattle.
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