Evidence map›Paper›PMID 34439765›Full record

ReviewBiomolecules2021

Oxidative Power: Tools for Assessing LPMO Activity on Cellulose.

Federica Calderaro, Loes E Bevers, Marco A van den Berg

Abstract readReview
In one paragraph

Review in Biomolecules, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Federica CalderaroDSM Biotechnology Center, 2613 AX Delft, The Netherlands.ORCID 0000-0003-1962-0018
Loes E BeversDSM Biotechnology Center, 2613 AX Delft, The Netherlands.
Marco A van den BergDSM Biotechnology Center, 2613 AX Delft, The Netherlands.ORCID 0000-0003-2770-9944

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lytic polysaccharide monooxygenases (LPMOs) have sparked a lot of research regarding their fascinating mode-of-action. Particularly, their boosting effect on top of the well-known cellulolytic enzymes in lignocellulosic hydrolysis makes them industrially relevant targets. As more characteristics of LPMO and its key role have been elucidated, the need for fast and reliable methods to assess its activity have become clear. Several aspects such as its co-substrates, electron donors, inhibiting factors, and the inhomogeneity of lignocellulose had to be considered during experimental design and data interpretation, as they can impact and often hamper outcomes. This review provides an overview of the currently available methods to measure LPMO activity, including their potential and limitations, and it is illustrated with practical examples.

Indexed as

BiomassCelluloseChromatographyChromatography, High Pressure LiquidFluorescence Resonance Energy TransferFungal ProteinsFungiHydrolysisKineticsLigninMagnetic Resonance ImagingMixed Function OxygenasesOxidation-ReductionOxidative StressPhotoelectron SpectroscopyPolysaccharidesCelluloseFungal ProteinsLigninlignocelluloseMixed Function OxygenasesPolysaccharidescellulosechromatographyenzyme assayenzyme kineticslignocellulosic biomasslytic polysaccharide monooxygenase

Identifiers

PMID34439765
PMCPMC8391687

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.