ArticleMicrobial biotechnology2022
Mining and engineering exporters for titer improvement of macrolide biopesticides in Streptomyces.
Article in Microbial biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Enhancing gougerotin production by screening endogenous promoters for the transporter geneFrontiers in microbiology · 2025Article
- A previously unidentified sugar transporter for engineering of high-yield Streptomyces.Applied microbiology and biotechnology · 2024Article
- Influence of Cluster-Situated Regulator PteF in Filipin Biosynthetic Cluster on Avermectin Biosynthesis inBiology · 2024Article
- Protein Modelling Highlighted Key Catalytic Sites Involved in Position-Specific Glycosylation of Isoflavonoids.International journal of molecular sciences · 2023Article
- New insight into the production improvement and resource generation of chaetoglobosin A in Chaetomium globosum.Microbial biotechnology · 2022Article
- Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux.Synthetic and systems biotechnology · 2022Article
- Comparative Transcriptome-Based Mining of Genes Involved in the Export of Polyether Antibiotics for Titer Improvement.Antibiotics (Basel, Switzerland) · 2022Article
- Mining and engineering exporters for titer improvement of macrolide biopesticides in Streptomyces.Microbial biotechnology · 2022Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exporter engineering is a promising strategy to construct high-yield Streptomyces for natural product pharmaceuticals in industrial biotechnology. However, available exporters are scarce, due to the limited knowledge of bacterial transporters. Here, we built a workflow for exporter mining and devised a tunable plug-and-play exporter (TuPPE) module to improve the production of macrolide biopesticides in Streptomyces. Combining genome analyses and experimental confirmations, we found three ATP-binding cassette transporters that contribute to milbemycin production in Streptomyces bingchenggensis. We then optimized the expression level of target exporters for milbemycin titer optimization by designing a TuPPE module with replaceable promoters and ribosome binding sites. Finally, broader applications of the TuPPE module were implemented in industrial S. bingchenggensis BC04, Streptomyces avermitilis NEAU12 and Streptomyces cyaneogriseus NMWT1, which led to optimal titer improvement of milbemycin A3/A4, avermectin B
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