ArticleThe Plant cell2021
Immunocapture of dsRNA-bound proteins provides insight into Tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Accelerating Resistance Breeding: Emerging Methods to Identify and Validate Plant Immunity Genes.Plants (Basel, Switzerland) · 2026Review
- Viral tropism in plants, reproductive tissues, and seeds.Archives of microbiology · 2025Review
- Rice JASMONIC ACID OXIDASES control resting jasmonate metabolism to promote growth and repress basal immune responses.Plant physiology · 2025Article
- Deficiency of multiple RNA silencing-associated genes may contribute to the increased susceptibility of Nicotiana benthamiana to viruses.Plant cell reports · 2024Article
- Mutations in the WG and GW motifs of the three RNA silencing suppressors of grapevine fanleaf virus alter their systemic suppression ability and affect virus infectivity.Frontiers in microbiology · 2024Article
- Co-immunoprecipitation-Based Isolation of Double-Stranded RNA-Associated Protein Complexes in Nicotiana benthamiana.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Salicylic acid and RNA interference mediate antiviral immunity of plant stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- dsRNA-induced immunity targets plasmodesmata and is suppressed by viral movement proteins.The Plant cell · 2023Article
- Properties of Plant Virus Protein Encoded by the 5'-Proximal Gene of Tetra-Cistron Movement Block.International journal of molecular sciences · 2023Article
- Proteomics-based determination of double-stranded RNA interactome reveals known and new factors involved in Sindbis virus infection.RNA (New York, N.Y.) · 2023Article
- Silencing ofPlant signaling & behavior · 2022Article
- Bioinformatic Analysis Predicts a Novel Genetic Module Related to Triple Gene and Binary Movement Blocks of Plant Viruses: Tetra-Cistron Movement Block.Biomolecules · 2022Article
- RNAi-Based Antiviral Innate Immunity in Plants.Viruses · 2022Review
- Atypical molecular features of RNA silencing against the phloem-restricted polerovirus TuYV.Nucleic acids research · 2021Article
- Characterization of a DCL2-InsensitiveViruses · 2020Article
- Double-Stranded-RNA-Binding Protein 2 Participates in Antiviral Defense.Journal of virology · 2020Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant RNA viruses form organized membrane-bound replication complexes to replicate their genomes. This process requires virus- and host-encoded proteins and leads to the production of double-stranded RNA (dsRNA) replication intermediates. Here, we describe the use of Arabidopsis thaliana expressing GFP-tagged dsRNA-binding protein (B2:GFP) to pull down dsRNA and associated proteins in planta upon infection with Tobacco rattle virus (TRV). Mass spectrometry analysis of the dsRNA-B2:GFP-bound proteins from infected plants revealed the presence of viral proteins and numerous host proteins. Among a selection of nine host candidate proteins, eight showed relocalization upon infection, and seven of these colocalized with B2-labeled TRV replication complexes. Infection of A. thaliana T-DNA mutant lines for eight such factors revealed that genetic knockout of dsRNA-BINDING PROTEIN 2 (DRB2) leads to increased TRV accumulation and DRB2 overexpression caused a decrease in the accumulation of four different plant RNA viruses, indicating that DRB2 has a potent and wide-ranging antiviral activity. We propose B2:GFP-mediated pull down of dsRNA to be a versatile method to explore virus replication complex proteomes and to discover key host virus replication factors. Given the universality of dsRNA, development of this tool holds great potential to investigate RNA viruses in other host organisms.
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