ArticleJournal of experimental botany2022
Complexome profiling on the Chlamydomonas lpa2 mutant reveals insights into PSII biogenesis and new PSII associated proteins.
Article in Journal of experimental botany, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 28 citations in OpenAlex.
- Complexome profiling of the Chlamydomonas psb28 mutant reveals TEF5 as an early PSII assembly factor.The Plant cell · 2025Article
- Photoreceptor-induced LHL4 protects the photosystem II monomer inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Effects of exogenous melatonin on the growth and photosynthetic characteristics of tomato seedlings under saline-alkali stress.Scientific reports · 2025Article
- Structural basis for an early stage of the photosystem II repair cycle in Chlamydomonas reinhardtii.Nature communications · 2024Article
- Assembly and Repair of Photosystem II inPlants (Basel, Switzerland) · 2024Review
- CLPB3 is required for the removal of chloroplast protein aggregates and thermotolerance in Chlamydomonas.Journal of experimental botany · 2023Article
- One-helix protein 2 is not required for the synthesis of photosystem II subunit D1 in Chlamydomonas.Plant physiology · 2023Article
- Structure of native photosystem II assembly intermediate fromFrontiers in plant science · 2023Article
- New destination vectors facilitate Modular Cloning for Chlamydomonas.Current genetics · 2022Article
- Article
- Fatty acid export (FAX) proteins contribute to oil production in the green microalgaFrontiers in molecular biosciences · 2022Article
- Molecular Advancements Establishing Chlamydomonas as a Host for Biotechnological Exploitation.Frontiers in plant science · 2022Review
- Discovery of photosynthesis genes through whole-genome sequencing of acetate-requiring mutants of Chlamydomonas reinhardtii.PLoS genetics · 2021Article
- LPA2 protein is involved in photosystem II assembly in Chlamydomonas reinhardtii.The Plant journal : for cell and molecular biology · 2021Article
- Complexome Profiling-Exploring Mitochondrial Protein Complexes in Health and Disease.Frontiers in cell and developmental biology · 2021Review
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the composition and function of the major thylakoid membrane complexes are well understood, comparatively little is known about their biogenesis. The goal of this work was to shed more light on the role of auxiliary factors in the biogenesis of photosystem II (PSII). Here we have identified the homolog of LOW PSII ACCUMULATION 2 (LPA2) in Chlamydomonas. A Chlamydomonas reinhardtii lpa2 mutant grew slower in low light, was hypersensitive to high light, and exhibited aberrant structures in thylakoid membrane stacks. Chlorophyll fluorescence (Fv/Fm) was reduced by 38%. Synthesis and stability of newly made PSII core subunits D1, D2, CP43, and CP47 were not impaired. However, complexome profiling revealed that in the mutant CP43 was reduced to ~23% and D1, D2, and CP47 to ~30% of wild type levels. Levels of PSI and the cytochrome b6f complex were unchanged, while levels of the ATP synthase were increased by ~29%. PSII supercomplexes, dimers, and monomers were reduced to ~7%, ~26%, and ~60% of wild type levels, while RC47 was increased ~6-fold and LHCII by ~27%. We propose that LPA2 catalyses a step during PSII assembly without which PSII monomers and further assemblies become unstable and prone to degradation. The LHCI antenna was more disconnected from PSI in the lpa2 mutant, presumably as an adaptive response to reduce excitation of PSI. From the co-migration profiles of 1734 membrane-associated proteins, we identified three novel putative PSII associated proteins with potential roles in regulating PSII complex dynamics, assembly, and chlorophyll breakdown.
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