Evidence map›Paper›PMID 34436264›Full record

ArticleMarine drugs2021

Identification of a New Antimicrobial, Desertomycin H, Utilizing a Modified Crowded Plate Technique.

Osama G Mohamed, Sadaf Dorandish, Rebecca Lindow, Megan Steltz, Ifrah Shoukat, Maira Shoukat, Hussein Chehade, Sara Baghdadi, Madelaine McAlister-Raeburn, Asad Kamal and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Marine drugs, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Osama G MohamedNatural Products Discovery Core, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0003-3216-6948
Sadaf DorandishBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Rebecca LindowBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Megan SteltzBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.ORCID 0000-0003-1125-8181
Ifrah ShoukatBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Maira ShoukatBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Hussein ChehadeBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Sara BaghdadiBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Madelaine McAlister-RaeburnBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Asad KamalBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Dawit AbebeBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Khaled AliBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Chelsey IvyBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Maria AntonovaBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Pamela SchultzNatural Products Discovery Core, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.
Michael AngellBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.ORCID 0000-0001-5024-3666
Daniel ClemansBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Timothy FriebeChemistry Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
David ShermanLife Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.
Anne M CasperBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Paul A PriceBiology Department, Eastern Michigan University, Ypsilanti, MI 48197, USA.
Ashootosh TripathiNatural Products Discovery Core, Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA.ORCID 0000-0003-2297-8088

Funding

University of Michigan Biosciences Initiative NA
6 · The paper itself

Abstract

The antibiotic-resistant bacteria-associated infections are a major global healthcare threat. New classes of antimicrobial compounds are urgently needed as the frequency of infections caused by multidrug-resistant microbes continues to rise. Recent metagenomic data have demonstrated that there is still biosynthetic potential encoded in but transcriptionally silent in cultivatable bacterial genomes. However, the culture conditions required to identify and express silent biosynthetic gene clusters that yield natural products with antimicrobial activity are largely unknown. Here, we describe a new antibiotic discovery scheme, dubbed the modified crowded plate technique (mCPT), that utilizes complex microbial interactions to elicit antimicrobial production from otherwise silent biosynthetic gene clusters. Using the mCPT as part of the antibiotic crowdsourcing educational program Tiny Earth

Indexed as

AnimalsAnti-Bacterial AgentsAquatic OrganismsCrowdsourcingMacrolidesAnti-Bacterial AgentsdesertomycinMacrolidesantibiotic discoverycrowded plate techniqueESKAPE pathogensnatural productsTiny EarthTM

Identifiers

PMID34436264
PMCPMC8400312

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.