Evidence map›Paper›PMID 34435949›Full record

ArticleeLife2021

Disintegration promotes protospacer integration by the Cas1-Cas2 complex.

Chien-Hui Ma, Kamyab Javanmardi, Ilya J Finkelstein, Makkuni Jayaram

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Chien-Hui MaDepartment of Molecular Biosciences and Institute of Cell and Molecular Biology, University of Texas at Austin, Austin, United States.
Kamyab JavanmardiDepartment of Molecular Biosciences and Institute of Cell and Molecular Biology, University of Texas at Austin, Austin, United States.ORCID 0000-0002-6449-6709
Ilya J FinkelsteinDepartment of Molecular Biosciences and Institute of Cell and Molecular Biology, University of Texas at Austin, Austin, United States.ORCID 0000-0002-9371-2431
Makkuni JayaramDepartment of Molecular Biosciences and Institute of Cell and Molecular Biology, University of Texas at Austin, Austin, United States.ORCID 0000-0001-9640-6264
The University of Texas at Austin · US

Funding

Mechanism, specificity, and design of CRISPR RNA-mediated gene regulationR01GM124141 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI FINKELSTEIN, ILYA J · 2017 to 2020
$1.2M
NIGMS NIH HHS R01 GM124141
6 · The paper itself

Abstract

'Disintegration'-the reversal of transposon DNA integration at a target site-is regarded as an abortive off-pathway reaction. Here, we challenge this view with a biochemical investigation of the mechanism of protospacer insertion, which is mechanistically analogous to DNA transposition, by the

Indexed as

Bacterial ProteinsClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated ProteinsEndonucleasesStreptococcus pyogenesBacterial ProteinsCRISPR-Associated ProteinsEndonucleasesadaptationbiochemistrychemical biologyCRISPRE. colitransposition

Identifiers

PMID34435949
PMCPMC8390005
OpenAlexW3193847231

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.