Evidence map›Paper›PMID 34435306›Full record

ArticlePharmaceutical research2021

Model Analysis of the Apparent Saturation Kinetics of Purine Nucleobase Uptake in Cells co-Expressing Transporter and Metabolic Enzyme.

Satoru Suzuki, Katsuhisa Inoue, Ikumi Tamai, Yoshiyuki Shirasaka

Abstract read
PubMed Publisher
In one paragraph

Article in Pharmaceutical research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
  2. Inborn Errors of Nucleoside Transporter (NT)-Encoding Genes (International journal of molecular sciences · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Satoru SuzukiFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.
Katsuhisa InoueSchool of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Tokyo, 192-0392, Japan.
Ikumi TamaiFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan.
Yoshiyuki ShirasakaFaculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan. shira@p.kanazawa-u.ac.jp.ORCID http://orcid.org/0000-0003-1472-7449
Kanazawa University · JPTokyo University of Pharmacy and Life Sciences · JP

Funding

Japan Society for the Promotion of Science 20K07165
6 · The paper itself

Abstract

purposeThis study aims to understand the effect of salvage enzyme activity on the saturable kinetics of facilitated cellular uptake of purine nucleobase by developing a cellular kinetic model incorporating equilibrative nucleobase transporter 1 (ENBT1) and adenine phosphoribosyltransferase (APRT), with adenine as a model nucleobase.

methodsA cellular kinetic model incorporating the functions of ENBT1 and APRT was developed using Napp software and employed for model-based analysis of the cellular disposition of adenine.

resultsSimulation analysis using the developed cellular kinetic model could account for the experimentally observed time-dependent changes in the K

conclusionOur findings indicate that both enzyme expression levels and experimental time should be considered when using cellular uptake studies to determine the K

Indexed as

AdenineAnimalsBiological TransportCell LineDogsEquilibrative Nucleoside Transport ProteinsKineticsMadin Darby Canine Kidney CellsPurinesAdenineEquilibrative Nucleoside Transport ProteinspurinePurinesAdenineadenine phosphoribosyltransferase (APRT)Equilibrative nucleobase transporter 1 (ENBT1)modeling & simulation (M&S)uptake study

Identifiers

PMID34435306
OpenAlexW3194099088

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.