ArticleBiology open2021
BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo.
Article in Biology open, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- Interpretable decoding of cell fate from a snapshot of combinatorial signaling.bioRxiv : the preprint server for biology · 2026Article
- Dependence of cell fate potential and cadherin switching on the coordinate within the primitive streak during differentiation of human pluripotent stem cells.Development (Cambridge, England) · 2026Article
- Amniogenesis in embryos and stem cell models.Nature cell biology · 2026Review
- Article
- Juxtaposition of human pluripotent stem cells with amnion-like cells is sufficient to trigger primitive streak formation.bioRxiv : the preprint server for biology · 2025Article
- Pluripotent cell states and fates in human embryo models.Development (Cambridge, England) · 2025Review
- A comprehensive human embryo reference tool using single-cell RNA-sequencing data.Nature methods · 2025Article
- Primate amnion development.Development (Cambridge, England) · 2024Review
- Highly efficient generation of self-renewing trophoblast from human pluripotent stem cells.iScience · 2024Article
- Combinatorial interpretation of BMP and WNT controls the decision between primitive streak and extraembryonic fates.Cell systems · 2024Article
- Human surface ectoderm and amniotic ectoderm are sequentially specified according to cellular density.Science advances · 2024Article
- Article
- Using Embryo Models to Understand the Development and Progression of Embryonic Lineages: A Focus on Primordial Germ Cell Development.Cells, tissues, organs · 2024Review
- Signaling mechanisms that direct cell fate specification and morphogenesis in human embryonic stem cells-based models of human gastrulation.Emerging topics in life sciences · 2023Review
- The many dimensions of germline competence.Current opinion in cell biology · 2023Review
- Review
- Expanded Potential Stem Cells from Human Embryos Have an Open Chromatin Configuration with Enhanced Trophoblast Differentiation Ability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Progress of Wnt Signaling Pathway in Osteoporosis.Biomolecules · 2023Review
- Human early syncytiotrophoblasts are highly susceptible to SARS-CoV-2 infection.Cell reports. Medicine · 2022Article
- Monosomy X in isogenic human iPSC-derived trophoblast model impacts expression modules preserved in human placenta.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human embryonic stem cells (hESCs) possess an immense potential to generate clinically relevant cell types and unveil mechanisms underlying early human development. However, using hESCs for discovery or translation requires accurately identifying differentiated cell types through comparison with their in vivo counterparts. Here, we set out to determine the identity of much debated BMP-treated hESCs by comparing their transcriptome to recently published single cell transcriptomic data from early human embryos ( Xiang et al., 2020). Our analyses reveal several discrepancies in the published human embryo dataset, including misclassification of putative amnion, intermediate and inner cell mass cells. These misclassifications primarily resulted from similarities in pseudogene expression, highlighting the need to carefully consider gene lists when making comparisons between cell types. In the absence of a relevant human dataset, we utilized the recently published single cell transcriptome of the early post implantation monkey embryo to discern the identity of BMP-treated hESCs. Our results suggest that BMP-treated hESCs are transcriptionally more similar to amnion cells than trophectoderm cells in the monkey embryo. Together with prior studies, this result indicates that hESCs possess a unique ability to form mature trophectoderm subtypes via an amnion-like transcriptional state. This article has an associated First Person interview with the first author of the paper.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.