Evidence map›Paper›PMID 34434735›Full record

ArticleMethodsX2021

Correcting for enzyme immunoassay changes in long term monitoring studies.

Abbey E Wilson, Agnieszka Sergiel, Nuria Selva, Jon E Swenson, Andreas Zedrosser, Gordon Stenhouse, David M Janz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Abbey E WilsonDepartment of Veterinary Biomedical Sciences, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.
Agnieszka SergielInstitute of Nature Conservation, Polish Academy of Sciences, Adama Mickiewicza 33, 31120 Krakow, Poland.
Nuria SelvaInstitute of Nature Conservation, Polish Academy of Sciences, Adama Mickiewicza 33, 31120 Krakow, Poland.
Jon E SwensonFaculty of Environmental Science and Natural Resource Management, Norwegian University of Life Sciences, Høgskoleveien 12, NO-1432, Norway.
Andreas ZedrosserDepartment of Natural Sciences and Environmental Health, University of South-Eastern Norway, Gullbringvegen 36, 3800 Bø, Norway.
Gordon StenhousefRI Research, Grizzly Bear Program, 1176 Switzer Drive, Hinton T7V 1V3, AB, Canada.
David M JanzDepartment of Veterinary Biomedical Sciences, University of Saskatchewan, 44 Campus Drive, Saskatoon, SK S7N 5B3, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzyme immunoassays (EIAs) are a common tool for measuring steroid hormones in wildlife due to their low cost, commercial availability, and rapid results. Testing technologies improve continuously, sometimes requiring changes in protocols or crucial assay components. Antibody replacement between EIA kits can cause differences in EIA sensitivity, which can hinder monitoring hormone concentration over time. The antibody in a common cortisol EIA kit used for long-term monitoring of stress in wildlife was replaced in 2014, causing differences in cross reactivity and standard curve concentrations. Therefore, the objective of this study was to develop a method to standardize results following changes in EIA sensitivity. We validated this method using cortisol concentrations measured in the hair of brown bears (

Indexed as

Brown bearEnzyme immunoassayHair cortisolStandardizationWildlife

Identifiers

PMID34434735
PMCPMC8374155

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.