ArticleCommunications biology2021
Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Target discrimination and PAM profiling of the Thermotoga maritima type I-B CRISPR system.The Biochemical journal · 2026Article
- Optimization of PHBV Synthesis Using Acetate as a Carbon Source by Paracoccus shandongensis wg2 via Response Surface Methodology.Current microbiology · 2026Article
- Recent Advances in Understanding Bio-Compounds from Haloarchaea.Journal of microbiology and biotechnology · 2026Review
- Microbial conversion of biodiesel waste for carotenoid production.Frontiers in bioengineering and biotechnology · 2026Article
- Advanced Strategies for Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Production: PHA Synthase Homologous Overexpression in the ExtremophileMarine drugs · 2025Article
- Bioplastic Production from Agri-Food Waste through the Use ofMicroorganisms · 2024Review
- Natural Polyhydroxyalkanoates-An Overview of Bacterial Production Methods.Molecules (Basel, Switzerland) · 2024Review
- Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications.Frontiers in microbiology · 2023Review
- Reprogramming the endogenous type I CRISPR-Cas system for simultaneous gene regulation and editing inmLife · 2022Article
- Article
- Study on the production of high 3HV content PHBVFrontiers in microbiology · 2022Article
- Current Advances towards 4-Hydroxybutyrate Containing Polyhydroxyalkanoates Production for Biomedical Applications.Molecules (Basel, Switzerland) · 2021Review
- Microbial-Derived Polyhydroxyalkanoate-Based Scaffolds for Bone Tissue Engineering: Biosynthesis, Properties, and Perspectives.Frontiers in bioengineering and biotechnology · 2021Review
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The haloarchaeon Haloferax mediterranei is a potential strain for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production, yet the production yield and cost are the major obstacles hindering the use of this archaeal strain. Leveraging the endogenous type I-B CRISPR-Cas system in H. mediterranei, we develop a CRISPR-based interference (CRISPRi) approach that allows to regulate the metabolic pathways related to PHBV synthesis, thereby enhancing PHBV production. Our CRISPRi approach can downregulate the gene expression in a range of 25% to 98% depending upon the target region. Importantly, plasmid-mediated CRISPRi downregulation on the citrate synthase genes (citZ and gltA) improves the PHBV accumulation by 76.4% (from 1.78 to 3.14 g/L). When crRNA cassette integrated into chromosome, this further shortens the PHBV fermentation period and enhances PHA productivity by 165%. Our transcriptome analysis shows that repression of citrate synthase genes redirects metabolic flux from the central metabolic pathways to PHBV synthesis pathway. These findings demonstrate that the CRISPRi-based gene regulation is a transformative toolkit for fine-tuning the endogenous metabolic pathways in the archaeal system, which can be applied to not only the biopolymer production but also many other applications.
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