ArticleBMC infectious diseases2021
Development and application of a method to detect 27 respiratory pathogens using multiplex RT-PCR combined with MassARRAY technology.
Article in BMC infectious diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- [Overview of PCR-based Diagnostic Assays for Emerging Infectious Disease Pathogens].Jugan geon-gang gwa jilbyeong · 2025Review
- An Aptamer-Based gFET-Sensor for Specific Quantification of Gene Therapeutic Human Adenovirus Type 5.Biosensors · 2025Article
- Development of a high-throughput MassARRAY-based single assay for the characterization of Streptococcus suis species and serotypes.Scientific reports · 2025Article
- Rapid authentication of endangeredFrontiers in plant science · 2025Article
- Simultaneously ultrasensitive and differential detection of SARS-CoV-2, adenovirus and influenza a virus using multiplex fluorescence lateral flow immunoassay.Frontiers in immunology · 2025Article
- A comparative study using Xpert MTB/RIF and culture methods evaluates MassARRAY technology for rapid detection ofFrontiers in cellular and infection microbiology · 2025Article
- MALDI-TOF mass spectrometry from nucleic acid: development and evaluation of a novel platform for identification of mycobacteria and detection of genetic markers of resistance.Microbiology spectrum · 2024Article
- Advances in nucleic acid aptamer-based detection of respiratory virus and bacteria: a mini review.Virology journal · 2024Review
- MassARRAY: a high-throughput solution for rapid detection of foodborne pathogens in real-world settings.Frontiers in microbiology · 2024Article
- Roles for Pathogen Interference in Influenza Vaccination, with Implications to Vaccine Effectiveness (VE) and Attribution of Influenza Deaths.Infectious disease reports · 2022Review
- Application of MRT-qPCR for pathogen detection of lower respiratory tract infection.American journal of translational research · 2022Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRespiratory tract infections are the most common infections that lead to morbidity and mortality worldwide. Early recognition and precise diagnosis of microbial etiology is important to treat LRTIs promptly, specifically and effectively.
objectivesTo establish a method based on multiplex reverse transcription (MRT)-PCR and MassARRAY technology for the simultaneous detection of 27 respiratory pathogens and explore its clinical application value.
methodsAnalytical sensitivity and specificity of the MRT-PCR-MassARRAY system were validated using inactivated bacterial and viral strains. Also we analyzed samples from 207 patients by MassARRAY methods and compared the results with consensus PCR/reverse transcription (RT)-PCR.
resultsThe minimum detection limit of our MRT-PCR-MassARRAY method for pathogens was 10-100 copies/μl, with high specificity. Comparison test with consensus PCR/RT-PCR on 207 clinical samples, the positive, negative, and total correlation rates were 100, 98.68, and 99.03%, respectively. There was a high degree of agreement between the test results of the two methods (P < 0.01 by McNemar's test).
conclusionOur detection system of 27 respiratory pathogens based on MassARRAY technology has high sensitivity and specificity, high throughput, and is simple to operate. It provides diagnostic value for the clinical diagnosis of respiratory pathogens and is of great significance in the screening of respiratory pathogens.
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