ArticleArteriosclerosis, thrombosis, and vascular biology2021
Single-Cell RNA Sequencing Reveals Endothelial Cell Transcriptome Heterogeneity Under Homeostatic Laminar Flow.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A multi-dataset single-cell meta-analysis of human keloid skin across Asian, Black and White populations reveals endothelial and mesenchymal programs linked to ethnic disparities.Frontiers in medicine · 2026Pooled it
- Opportunities for RNA sequencing in physiology: from big data to understanding homeostasis and heterogeneity.Function (Oxford, England) · 2026Review
- Decoding Vascular Cell Diversity: Single-Cell Approaches to Mechanisms of Vascular Disease.Circulation research · 2026Review
- Cell-type-specific alkaloid and terpenoid biosynthesis in glandular trichomes: single-cell and spatial transcriptomic perspectives.Frontiers in plant science · 2026Review
- Increasing complexity in vascular microphysiological systems: considerations and advances for disease modeling.Frontiers in bioengineering and biotechnology · 2026Review
- Vascular-type heterogeneity is associated with differential gene expression profiles of endothelial cells under shear stress.Research and practice in thrombosis and haemostasis · 2025Article
- Biophysical and Biochemical Roles of Shear Stress on Endothelium: A Revisit and New Insights.Circulation research · 2025Review
- Dissecting endothelial cell heterogeneity with new tools.Cell regeneration (London, England) · 2025Review
- Review
- Impact of mechanical cues on key cell functions and cell-nanoparticle interactions.Discover nano · 2024Review
- Endothelial Cell Flow-Mediated Quiescence Is Temporally Regulated and Utilizes the Cell Cycle Inhibitor p27.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Article
- Mechanotransduction Circuits in Human Pathobiology.International journal of molecular sciences · 2024Article
- Single cell 'omic profiles of human aortic endothelial cellsbioRxiv : the preprint server for biology · 2024Article
- Endothelial cell flow-mediated quiescence is temporally regulated and utilizes the cell cycle inhibitor p27.bioRxiv : the preprint server for biology · 2024Article
- Capturing physiological hemodynamic flow and mechanosensitive cell signaling in vessel-on-a-chip platforms.Frontiers in physiology · 2024Review
- Monocyte adhesion to and transmigration through endothelium following cardiopulmonary bypass shearing is mediated by IL-8 signaling.Frontiers in cardiovascular medicine · 2024Article
- Integrated systems biology approach identifies gene targets for endothelial dysfunction.Molecular systems biology · 2023Article
- Cellular mechanotransduction in health and diseases: from molecular mechanism to therapeutic targets.Signal transduction and targeted therapy · 2023Review
- Regeneration of tracheal neotissue in partially decellularized scaffolds.NPJ Regenerative medicine · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Objective: Endothelial cells (ECs) that form the innermost layer of all vessels exhibit heterogeneous cell behaviors and responses to pro-angiogenic signals that are critical for vascular sprouting and angiogenesis. Once vessels form, remodeling and blood flow lead to EC quiescence, and homogeneity in cell behaviors and signaling responses. These changes are important for the function of mature vessels, but whether and at what level ECs regulate overall expression heterogeneity during this transition is poorly understood. Here, we profiled EC transcriptomic heterogeneity, and expression heterogeneity of selected proteins, under homeostatic laminar flow. Approach and Results: Single-cell RNA sequencing and fluorescence microscopy were used to characterize heterogeneity in RNA and protein gene expression levels of human ECs under homeostatic laminar flow compared to nonflow conditions. Analysis of transcriptome variance, Gini coefficient, and coefficient of variation showed that more genes increased RNA heterogeneity under laminar flow relative to genes whose expression became more homogeneous, although small subsets of cells did not follow this pattern. Analysis of a subset of genes for relative protein expression revealed little congruence between RNA and protein heterogeneity changes under flow. In contrast, the magnitude of expression level changes in RNA and protein was more coordinated among ECs in flow versus nonflow conditions. Conclusions: ECs exposed to homeostatic laminar flow showed overall increased heterogeneity in RNA expression levels, while expression heterogeneity of selected cognate proteins did not follow RNA heterogeneity changes closely. These findings suggest that EC homeostasis is imposed post-transcriptionally in response to laminar flow.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.