ArticlePLoS neglected tropical diseases2021
Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera.
Article in PLoS neglected tropical diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 19 citations in OpenAlex.
- One-pot nanoflower-based sensitive colorimetric biosensor for multihost detection of zoonotic clonorchiasis.PLoS neglected tropical diseases · 2026Article
- Assessing diagnostic, vaccine and therapeutic potential of selectedFood and waterborne parasitology · 2025Review
- Early diagnosis ofVeterinary world · 2024Article
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- Identification of antigens in the Trichinella spiralis extracellular vesicles for serological detection of early stage infection in swine.Parasites & vectors · 2023Article
- The Prevalence ofAnimals : an open access journal from MDPI · 2022Article
- Hematological, biochemical, and serological parameters of experimentally infected rabbits withVeterinary world · 2022Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesTrichinella spiralis is a zoonotic parasite with a complex parasitic life cycle and exposed to animals or humans by infectious meat. To control transmissions of T. spiralis through the food chain to humans, sensitive and selective multihost sera-diagnosis is urgent needed for monitoring T. spiralis exposure.
methodsA competition enzyme-linked immunosorbent assay (cELISA) for T. spiralis infection diagnosis in multihost sera was developed based on recombinant cystatin-like protein (rCLP-cELISA) as well as monoclonal antibodies. The sensitivity and accuracy of the rCLP-cELISA were quantified using swine (n = 1316), mice (n = 189) and human (n = 157) serum samples. T. spiralis-antibody targeting test ability of the rCLP-cELISA in swine (n = 22) and human (n = 36), instead of other parasites or viruses antibodies, was evaluated.
resultsThe rCLP-cELISA showed high agreement with commercial ELISA kits in field swine sera assessed by Cohen's kappa value (κ = 0.7963). And it showed 100% specificity in human trichinellosis detection with sensitivity of 96.49%, no cross-reaction with other parasite or virus infections, and high positive detection rate of 87.5% in low-dose infected swine. Besides, the rCLP-cELISA exhibited potential in the detection of T. spiralis, T. nelsoni and Trichinella T8 infections.
conclusionsThe rCLP-cELISA can be used for T. spiralis-associated antibody test in multihost sera.
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