ArticleMolecular biology of the cell2021
Exploiting differential effects of actomyosin contractility to control cell sorting among breast cancer cells.
Article in Molecular biology of the cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Cyclic stretch inhibits cell invasion in 3D scaffolds.bioRxiv : the preprint server for biology · 2026Article
- Role of atomic force microscopy in characterization of heterotypic cancer spheroids and their interaction with microplastic particles.Scientific reports · 2026Article
- Metabolic starvation-induced cell swelling drives solid stress in tumors.bioRxiv : the preprint server for biology · 2026Article
- Anisotropy and shear stress accumulation during collective migration of epithelial cells.European biophysics journal : EBJ · 2026Review
- Brillouin Microscopy of Breast tumor Spheroids On-a-Chip: Mechanical and Transcriptional Responses to Microfluidic-Induced Rapid Deformations.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Review
- Restraint of cancer cell plasticity by spatial homotypic clustering.Cancer cell · 2025Article
- A microfluidic rheometer for tumor mechanics and invasion studies.Lab on a chip · 2025Article
- Aquaporin 5 expression regulates MDA-MB-231 spheroid multicellular invasion.American journal of physiology. Cell physiology · 2025Article
- A microfluidic rheometer for tumor mechanics and invasion studies.bioRxiv : the preprint server for biology · 2025Article
- Mechanical cues guide the formation and patterning of 3D spheroids in fibrous environments.PNAS nexus · 2025Article
- Clustered macrophages cooperate to eliminate tumors via coordinated intrudopodia.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Targeted elimination of mesenchymal-like cancer cells through cyclic stretch activation of Piezo1 channels: the physical aspects.Biophysical reviews · 2025Review
- Entropy Production in Epithelial Monolayers Due to Collective Cell Migration.Entropy (Basel, Switzerland) · 2025Review
- Friction in soft biological systems and surface self-organization: the role of viscoelasticity.Biophysical reviews · 2024Review
- Physical aspects of epithelial cell-cell interactions: hidden system complexities.European biophysics journal : EBJ · 2024Review
- Epithelial cell-cell interactions in an overcrowded environment: jamming or live cell extrusion.Journal of biological engineering · 2024Review
- Different contractility modes control cell escape from multicellular spheroids and tumor explants.APL bioengineering · 2024Article
- Role of viscoelasticity in the appearance of low-Reynolds turbulence: considerations for modelling.Journal of biological engineering · 2024Review
- Using Biosensors to Study Organoids, Spheroids and Organs-on-a-Chip: A Mechanobiology Perspective.Biosensors · 2023Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
In order to gain a greater understanding of the factors that drive spatial organization in multicellular aggregates of cancer cells, we investigate the segregation patterns of 6 breast cell lines of varying degree of mesenchymal character during formation of mixed aggregates. Cell sorting is considered in the context of available adhesion proteins and cellular contractility. It is found that the primary compaction mediator (cadherins or integrins) for a given cell type in isolation plays an important role in compaction speed, which in turn is the major factor dictating preference for interior or exterior position within mixed aggregates. In particular, cadherin-deficient, invasion-competent cells tend to position towards the outside of aggregates, facilitating access to extracellular matrix. Reducing actomyosin contractility is found to have a differential effect on spheroid formation depending on compaction mechanism. Inhibition of contractility has a significant stabilizing effect on cell-cell adhesions in integrin-driven aggregation and a mildly destabilizing effect in cadherin-based aggregation. This differential response is exploited to statically control aggregate organization and dynamically rearrange cells in pre-formed aggregates. Sequestration of invasive cells in the interior of spheroids provides a physical barrier that reduces invasion in three-dimensional culture, revealing a potential strategy for containment of invasive cell types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.