ArticleJournal of clinical microbiology2021
Single-Amplicon Multiplex Real-Time Reverse Transcription-PCR with Tiled Probes To Detect SARS-CoV-2
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Article in Journal of clinical microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21citing papers in PubMed
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Who cites it
21 citing papers in PubMed.
- BioInnovate AI: A Machine Learning Platform for Rapid PCR Assay Design in Emerging Infectious Disease Diagnostics.Diagnostics (Basel, Switzerland) · 2025Article
- Validation and implementation of TaqMAMA RT-PCR for SARS-CoV-2 variant surveillance: experience from a high-volume setting.BMC infectious diseases · 2025Article
- Article
- Epidemiological Insights into the Omicron Outbreak via MeltArray-Assisted Real-Time Tracking of SARS-CoV-2 Variants.Viruses · 2023Article
- SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2023Article
- Investigation of Blood Plasma Viral Nucleocapsid Antigen as a Marker of Active Severe Acute Respiratory Syndrome Coronavirus 2 Omicron Variant Infection.Open forum infectious diseases · 2023Article
- SARS-CoV-2 genotyping and sequencing following a simple and economical RNA extraction and storage protocol.PloS one · 2023Article
- A straightforward one-step strategy for SARS-CoV-2 diagnosis and screening of variants of concern: a multicentre study.Memorias do Instituto Oswaldo Cruz · 2023Article
- High sensitivity SARS-CoV-2 detection using graphene oxide-multiplex qPCR.Analytica chimica acta · 2022Article
- Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants.Open forum infectious diseases · 2022Article
- Accessible and Adaptable Multiplexed Real-Time PCR Approaches to Identify SARS-CoV-2 Variants of Concern.Microbiology spectrum · 2022Article
- Rapid Detection and Characterization of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Omicron Variant in a Returning Traveler.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Identifying SARS-CoV-2 Variants of Concern through Saliva-Based RT-qPCR by Targeting Recurrent Mutation Sites.Microbiology spectrum · 2022Article
- SARS-CoV-2 Variants in Paraguay: Detection and Surveillance with an Economical and Scalable Molecular Protocol.Viruses · 2022Article
- Highly Sensitive Lineage Discrimination of SARS-CoV-2 Variants through Allele-Specific Probe PCR.Journal of clinical microbiology · 2022Article
- Identifying SARS-CoV-2 Variants of Concern through saliva-based RT-qPCR by targeting recurrent mutation sites.medRxiv : the preprint server for health sciences · 2022Article
- Surveillance of SARS-CoV-2 variants of concern by identification of single nucleotide polymorphisms in the spike protein by a multiplex real-time PCR.Journal of virological methods · 2022Article
- Article
- Advances in the Rapid Diagnostic of Viral Respiratory Tract Infections.Frontiers in cellular and infection microbiology · 2022Review
- Severe acute respiratory syndrome coronavirus 2 vaccine breakthrough infections: A single metro-based testing network experience.Frontiers in medicine · 2022Article
Corrections and comments
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Authors and funding
19 authors.
Ahmed Babiker *Emory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.ORCID 0000-0003-0578-4871
Katherine Immergluck *Emory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.
Samuel D StampferEmory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.
Anuradha RaoThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
Leda BassitThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
Max SuEmory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.
Vi NguyenEmory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.
Victoria StittleburgEmory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.
Jessica M IngersollEmory University School of Medicinegrid.471395.d, Department of Pathology and Laboratory Medicine, Atlanta, Georgia, USA.
Heath L BradleyEmory University School of Medicinegrid.471395.d, Department of Pathology and Laboratory Medicine, Atlanta, Georgia, USA.
Maud MavignerThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.ORCID 0000-0001-9898-317X
Nils SchoofThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
Colleen S KraftEmory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.ORCID 0000-0003-1757-8477
Ann ChahroudiThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.ORCID 0000-0001-7479-9546
Raymond F SchinaziThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.ORCID 0000-0002-6688-3614
Greg S MartinThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
Anne PiantadosiEmory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.ORCID 0000-0002-5942-1534
Wilbur A LamEmory University School of Medicinegrid.471395.d, Department of Pathology and Laboratory Medicine, Atlanta, Georgia, USA.
Jesse J WaggonerEmory University School of Medicinegrid.471395.d, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.ORCID 0000-0002-0244-5957
Funding
Technology Training and Dissemination CoreU54EB027690 · NIBIB · EMORY UNIVERSITY · PI Wilbur A Lam, GREGORY S MARTIN · 2018 to 2026
$96.1MImplementing a Maternal health and PRegnancy Outcomes Vision for Everyone (IMPROVE)UL1TR002378 · NCATS · EMORY UNIVERSITY · PI Andres J Garcia, Elizabeth O. Ofili · 2017 to 2026
$92.1MAmerican Asian Centers for Arbovirus Research and Enhanced Surveillance (A2CARES)U01AI151788 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI Josefina Coloma, Eva Harris · 2020 to 2026
$9.6MTraining a New Generation of VaccinologistsT32AI074492 · NIAID · EMORY UNIVERSITY · PI Rama Rao Amara, Benjamin A Lopman · 2009 to 2026
$6.4MDoris Duke Charitable Foundation (DDCF) Clinical Scientist Development Award 2019089Emory University Woodruff Health Sciences Center COVID-19 CURE AwardHHS | Centers for Disease Control and Prevention (CDC) contract 75D30121C10084HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID) T32AI074492HHS | NIH | National Institute of Biomedical Imaging and Bioengineering (NIBIB) U54 EB027690 02S1NCATS NIH HHS UL1 TR002378NIAID NIH HHS T32 AI074492NIAID NIH HHS U01 AI151788NIBIB NIH HHS U54 EB027690
Abstract
To provide an accessible and inexpensive method to surveil for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations, we developed a multiplex real-time reverse transcription-PCR (rRT-PCR) assay, the Spike single-nucleotide polymorphism (SNP) assay, to detect specific mutations in the
Indexed as
COVID-19SARS-CoV-2HumansMutationReal-Time Polymerase Chain ReactionReverse TranscriptionRNA, ViralRNA, ViralCOVID-19diagnosticsmolecular epidemiologyvariants
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.