ArticleAnalytical and bioanalytical chemistry2021
One-step time-resolved fluorescence microsphere immunochromatographic test strip for quantitative and simultaneous detection of DON and ZEN.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 72 citations in OpenAlex.
- Nanotechnology in aflatoxin management: emerging tools for rapid detection and post-harvest control in grains.World journal of microbiology & biotechnology · 2026Review
- Core-shell Au@4-MBA@Ag enhanced SERS immunochromatography for rapid and on-site detection of deoxynivalenol in animal feeds.Analytical and bioanalytical chemistry · 2025Article
- A Time-Resolved Fluorescent Microsphere Immunochromatographic Assay for Determination of Vitamin B12 in Infant Formula Milk Powder.Biosensors · 2025Article
- Technologies for detecting biological risk factors in agricultural products and their applications.Current research in food science · 2025Review
- A Novel Immunochromatographic Test Strip Using Lanthanide-Labeled Fluorescent Nanoparticles for the Serological Detection ofPathogens (Basel, Switzerland) · 2024Article
- Preparation and preliminary application of fluorescent microsphere test strips for feline parvovirus antibodies.BMC biotechnology · 2024Article
- A Portable Fluorescent Lateral Flow Immunoassay Platform for Rapid Detection of FluA.Biosensors · 2024Article
- Development of a time-resolved immunochromatographic test strip for rapid and quantitative determination of retinol-binding protein 4 in urine.Mikrochimica acta · 2024Article
- Development of a Time-Resolved Fluorescent Microsphere Test Strip for Rapid, On-Site, and Sensitive Detection of Picoxystrobin in Vegetables.Foods (Basel, Switzerland) · 2024Article
- Preparation of Anti-Zearalenone IgY and Development of an Indirect Competitive ELISA Method for the Measurement of Zearalenone in Post-Fermented Tea.Foods (Basel, Switzerland) · 2023Article
- Zeolitic Imidazolate Framework-8 Composite-Based Enzyme-Linked Aptamer Assay for the Sensitive Detection of Deoxynivalenol.Biosensors · 2023Article
- A novel dual-channel immunochromatographic strip using up-conversion nanoparticles for simultaneous detection of AFB1 and ZEN in maize.Analytical and bioanalytical chemistry · 2023Article
- Europium Nanoparticles-Based Fluorescence Immunochromatographic Detection of Three Abused Drugs in Hair.Toxics · 2023Article
- A time-resolved fluorescence microsphere-lateral flow immunochromatographic strip for quantitative detection of Pregnanediol-3-glucuronide in urine samples.Frontiers in bioengineering and biotechnology · 2023Article
- Biotin-labelled peptidomimetic for competitive time-resolved fluoroimmunoassay of benzothiostrobin.Analytical and bioanalytical chemistry · 2022Article
- Lateral Flow Immunoassay Based on Time-Resolved Fluorescence Microspheres for Rapid and Quantitative Screening CA199 in Human Serum.International journal of molecular sciences · 2022Article
- Novel Dual-Color Immunochromatographic Assay Based on Chrysanthemum-like Au@polydopamine and Colloidal Gold for Simultaneous Sensitive Detection of Paclobutrazol and Carbofuran in Fruits and Vegetables.Foods (Basel, Switzerland) · 2022Article
- A monoclonal antibody-based time-resolved fluorescence microsphere lateral flow immunoassay for paclobutrazol detection.Current research in food science · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Deoxynivalenol (DON) and zearalenone (ZEN) are mycotoxins that contaminate a wide range of grains and crops. In this study, a one-step time-resolved single-channel immunochromatographic test strip based on europium ion polystyrene fluorescence microspheres was first developed for sensitive and quantitative detection of DON and ZEN. The concentration of the artificial antigen and the mass ratio of the monoclonal antibody to fluorescent microspheres for conjugation were optimized to simplify the sample addition process during immunochromatographic assay and improve the on-site detection efficiency. The limits of detection (LOD) of the single-channel immunochromatographic test strip for DON and ZEN detection were 0.17 and 0.54 μg/L, respectively. Meanwhile, the dual-channel immunochromatographic test strip was designed to simultaneously detect DON and ZEN, with LODs of 0.24 and 0.69 μg/L achieved for DON and ZEN, respectively. The developed test strips also yielded recovery results consistent with that obtained by LC-MS/MS for DON and ZEN detection in real samples of wheat and corn flour, confirming the practicability and reliability of the test strip. The developed immunochromatographic test strips realize quick and sensitive detection of DON and ZEN, exhibiting potential for broad applications in the point-of-care testing platform of multiple mycotoxins in agricultural products. Graphic abstract.
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