Evidence map›Paper›PMID 34430913›Full record

ArticleSTAR protocols2021

A simple, quantitative assay for the detection of viable but non-culturable (VBNC) bacteria.

Ignacio Servando Arvizu, Sean Richard Murray

Abstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Persistence and Culturability ofAntibiotics (Basel, Switzerland) · 2024
    Article
  3. Article
  4. Expanding the microbiologist toolboxMicrobiology spectrum · 2024
    Article
  5. Article
  6. Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Ignacio Servando ArvizuDepartment of Biology, California State University, Northridge, 18111 Nordhoff Street, Northridge, CA 91330, USA.
Sean Richard MurrayDepartment of Biology, California State University, Northridge, 18111 Nordhoff Street, Northridge, CA 91330, USA.

Funding

Identification and characterization of factors affecting cytoskeletal proteins--the mediators of bacterial cell shapeSC3GM121234 · NIGMS · CALIFORNIA STATE UNIVERSITY NORTHRIDGE · PI MURRAY, SEAN · 2018 to 2021
$435k
NIGMS NIH HHS SC3 GM121234
6 · The paper itself

Abstract

This protocol determines the fraction of a bacterial population that is viable and culturable, viable and non-culturable, or non-viable (dead). Each population is detected by isolating colonies on agar plates, performing direct counts, and staining for live or dead cells. Its application is limited to bacteria that are stainable and when permissible growth conditions are known. The quantitative data extracted allow for the detection of a viable but non-culturable (alive and non-dividing) population from a liquid culture. For complete details on the use and execution of this protocol, please refer to Stott et al. (2015).

Indexed as

BacteriaMicrobial ViabilityMolecular Probe TechniquesBacteriological TechniquesMolecular ProbesStaining and LabelingMolecular ProbesCell-based AssaysCell BiologyMicrobiologyMicroscopy

Identifiers

PMID34430913
PMCPMC8365524

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.